Effects of Manganese Porphyrins on Cellular Sulfur Metabolism
| Autor: | Robert V. Stahelin, Michael D. Pluth, Ines Batinic-Haberle, Yan Gao, Karl D Straubg, David L. Boone, Andrea K. Steiger, Charles R. Tessier, Kenneth R. Olson, Troy A. Markel |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Porphyrins
Sulfur metabolism Pharmaceutical Science Ascorbic Acid Mitochondrion Article Analytical Chemistry lcsh:QD241-441 Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:Organic chemistry Drug Discovery Mn porphyrins Animals Humans Hydrogen Sulfide Physical and Theoretical Chemistry Hydrogen peroxide h2s 030304 developmental biology 0303 health sciences Manganese biology H2S Superoxide Dismutase Organic Chemistry ROS Metabolism Hydrogen Peroxide Ascorbic acid equipment and supplies Oxygen Cytosol antioxidants HEK293 Cells chemistry Chemistry (miscellaneous) reactive sulfide species biology.protein Biophysics Molecular Medicine SOD mimetics polysulfides Oxidation-Reduction 030217 neurology & neurosurgery Intracellular Sulfur |
| Zdroj: | Molecules Molecules, Vol 25, Iss 4, p 980 (2020) Volume 25 Issue 4 |
| ISSN: | 1420-3049 |
| Popis: | Manganese porphyrins (MnPs), MnTE-2-PyP5+, MnTnHex-2-PyP5+ and MnTnBuOE-2-PyP5+, are superoxide dismutase (SOD) mimetics and form a redox cycle between O2 and reductants, including ascorbic acid, ultimately producing hydrogen peroxide (H2O2). We previously found that MnPs oxidize hydrogen sulfide (H2S) to polysulfides (PS H2Sn, n = 2&ndash 6) in buffer. Here, we examine the effects of MnPs for 24 h on H2S metabolism and PS production in HEK293, A549, HT29 and bone marrow derived stem cells (BMDSC) using H2S (AzMC, MeRho-AZ) and PS (SSP4) fluorophores. All MnPs decreased intracellular H2S production and increased intracellular PS. H2S metabolism and PS production were unaffected by cellular O2 (5% versus 21% O2), H2O2 or ascorbic acid. We observed with confocal microscopy that mitochondria are a major site of H2S production in HEK293 cells and that MnPs decrease mitochondrial H2S production and increase PS in what appeared to be nucleoli and cytosolic fibrillary elements. This supports a role for MnPs in the metabolism of H2S to PS, the latter serving as both short- and long-term antioxidants, and suggests that some of the biological effects of MnPs may be attributable to sulfur metabolism. |
| Databáze: | OpenAIRE |
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