Participation of the histone-like protein HU and of IHF in minichromosomal maintenance in Escherichia coli
| Autor: | Hiraga Sota, Ogawa Tohru, Okazaki Tuneko, Kano Yasunobu, Imamoto Fumio, Ogura Teru |
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| Rok vydání: | 1991 |
| Předmět: |
DNA Replication
Integration Host Factors Protein subunit Mutant medicine.disease_cause Plasmid Bacterial Proteins Minichromosome Escherichia coli Genetics medicine Mutation biology General Medicine Chromosomes Bacterial biology.organism_classification Enterobacteriaceae Molecular biology DnaA DNA-Binding Proteins bacteria Transformation Bacterial Chromosome Deletion Plasmids |
| Zdroj: | Gene. 103:25-30 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(91)90386-p |
| Popis: | The closely related Escherichia coli genes, hupA, hupB, himA and himD (hip), encode the bacterial histone-like protein subunits, HU-2, HU-1, IHFα and IHFβ, respectively. We report here that E. coli minichromosomes [plasmids (2.7–12.2 kb) with oriC] carrying the intact mioC region were unable to transform mutants deficient in both HU and integration host factor (IHF), whereas they could transform mutants deficient in either HU or IHF as efficiently as the wild-type strain. Minichromosomes carrying a deletion of the proximal part of mioC or a DnaA box just upstream from mioC could not transform cells deficient in IHF, but could transform cells deficient in HU. These results suggested that HU and IHF participate in minichromosomal replication from oriC in E. coli. |
| Databáze: | OpenAIRE |
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