Crescent-Like Lesions as an Early Signature of Nephropathy in a Rat Model of Prediabetes Induced by a Hypercaloric Diet

Autor: Pedro Vieira, Flávio Reis, Sofia D. Viana, Sara Nunes, Inês Preguiça, Fernando Mendes, Adelaide Barbosa, André Alves, Diana Raquel Fernandes Martins
Přispěvatelé: Instituto de Investigação e Inovação em Saúde
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
Kidney Glomerulus
prediabetes
030204 cardiovascular system & hematology
Kidney Glomerulus / metabolism
Kidney Function Tests
diet-induced
Prediabetic State / complications
Diabetic nephropathy
Impaired glucose tolerance
Diabetic Nephropathies / etiology
0302 clinical medicine
Diet
Diabetic
Diabetic Nephropathies
Prediabetes
Diabetic Nephropathies / metabolism
Nutrition and Dietetics
Communication
Immunohistochemistry
nephropathy
lcsh:Nutrition. Foods and food supply
Collagen Type IV
medicine.medical_specialty
Kidney Glomerulus / pathology
Renal function
030209 endocrinology & metabolism
lcsh:TX341-641
Diabetic Nephropathies / pathology
Prediabetic State / metabolismo
Nephropathy
Prediabetic State
03 medical and health sciences
Diet
Diabetic / adverse effects
Internal medicine
Diabetes mellitus
Collagen Type IV / metabolism
medicine
Animals
business.industry
rat model
Hypertriglyceridemia
Body Weight
Prediabetic State / etiology
glomerular crescent-like lesions
medicine.disease
Lipid Metabolism
Rats
Disease Models
Animal
Endocrinology
renal lipidosis
business
Energy Intake
Biomarkers
Food Science
Kidney disease
Zdroj: Nutrients, Vol 12, Iss 4, p 881 (2020)
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação
instacron:RCAAP
Nutrients
ISSN: 2072-6643
Popis: Diabetic nephropathy (DN) is a major microvascular complication of diabetes. Obesity and hyperlipidemia, fueled by unhealthy food habits, are risk factors to glomerular filtration rate (GFR) decline and DN progression. Several studies recommend that diabetic patients should be screened early (in prediabetes) for kidney disease, in order to prevent advanced stages, for whom the current interventions are clearly inefficient. This ambition greatly depends on the existence of accurate early biomarkers and novel molecular targets, which only may arise with a more thorough knowledge of disease pathophysiology. We used a rat model of prediabetes induced by 23 weeks of high-sugar/high-fat (HSuHF) diet to characterize the phenotype of early renal dysfunction and injury. When compared with the control animals, HSuHF-treated rats displayed a metabolic phenotype compatible with obese prediabetes, displaying impaired glucose tolerance and insulin sensitivity, along with hypertriglyceridemia, and lipid peroxidation. Despite unchanged creatinine levels, the prediabetic animals presented glomerular crescent-like lesions, accompanied by increased kidney Oil-Red-O staining, triglycerides content and mRNA expression of IL-6 and iNOS. This model of HSuHF-induced prediabetes can be a useful tool to study early features of DN, namely crescent-like lesions, an early signature that deserves in-depth elucidation. This work was supported by the European Regional Development Fund (FEDER), through Programa Operacional Factores de Competitividade COMPETE2020 (CENTRO-01-0145-FEDER-000012-HealthyAging2020) and by National funds via Portuguese Science and Technology Foundation (FCT): Strategic Projects UID/NEU/04539/2013, UID/NEU/04539/2019, UIDB/04539/2020 and UIDP/04539/2020 (CIBB), SFRH/BD/109017/2015 (PhD Fellowship) and PTDC/SAU-NUT/31712/2017, as well as by COMPETE-FEDER funds (POCI-01-0145-FEDER-007440 and POCI-01-0145-FEDER-031712).
Databáze: OpenAIRE
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