Characterization of arabinogalactans from Larix principis-rupprechtii and their effects on NO production by macrophages
| Autor: | Caoxing Huang, Shuo Tang, Qiang Yong, Chenhuan Lai, Yimin Fan, Meiyun Jiang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Polymers and Plastics
Larix 02 engineering and technology Uronic acid Nitric Oxide Polysaccharide Galactans 01 natural sciences Gel permeation chromatography Mice chemistry.chemical_compound Arabinogalactan Materials Chemistry Animals Monosaccharide chemistry.chemical_classification Molecular mass biology 010405 organic chemistry GalP Macrophages Monosaccharides Organic Chemistry Nuclear magnetic resonance spectroscopy 021001 nanoscience & nanotechnology 0104 chemical sciences Molecular Weight RAW 264.7 Cells Carbohydrate Sequence chemistry Biochemistry biology.protein 0210 nano-technology |
| Zdroj: | Carbohydrate Polymers. 200:408-415 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2018.08.027 |
| Popis: | Arabinogalactans are a source of dietary fiber with health benefits. In this work, two arabinogalactans assigned as AGW and AGS were isolated from Larix principis-rupprechtii, and characterized by gel permeation chromatography (GPC), monosaccharide analysis, methylation analysis and NMR spectroscopy analysis. The average molecular weights of AGW and AGS were 1.53 × 104 and 1.84 × 104 Da, respectively. Methylation analysis and NMR spectra suggested that AGW and AGS have a 1,3-linked Galp backbone, branched at C-6 with 1,6-linked Galp side residues. The Ara residues were substituted at C-6 of 1,6-linked Galp consisting of α-L-Araf-(1→3)-α-L-Araf-(1→6)-β-D-Galp-(1→ and β-L-Arap-(1→6)-β-D-Galp-(1→. Significantly, AGS (0.74%) was shown to contain 25 times more uronic acid than AGW (0.03%), which demonstrated a polyelectrolyte effect. Application of these two polysaccharides to macrophage RAW 264.7 cells was shown to increase nitric oxide (NO) production. These results provide a basis for studying the relationship between the structure and biological activity of arabinogalactans. |
| Databáze: | OpenAIRE |
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