Expression of peroxisome proliferator-activated receptor-gamma in renal ischemia-reperfusion injury
| Autor: | Yutaka Kawahito, K. Tsuchida, Junji Uchida, Yoshiaki Takemoto, T. Nakatani, Masahide Matsuyama, Rikio Yoshimura, Taro Hase, Hajime Sano |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Pathology medicine.medical_specialty Endothelium Ischemia Kidney Renal Circulation medicine Animals Kidney Tubules Collecting Transplantation Renal circulation business.industry medicine.disease Immunohistochemistry Rats PPAR gamma medicine.anatomical_structure Rats Inbred Lew Renal physiology Reperfusion Injury Surgery Endothelium Vascular business Kidney disease |
| Zdroj: | Transplantation proceedings. 37(4) |
| ISSN: | 0041-1345 |
| Popis: | The pathogenesis of ischemia-reperfusion injury is known to involve cytokines and particularly surface adhesion molecules, the expression of which initiates the attachment of inflammatory cells. Peroxisome proliferator-activated receptor (PPAR)-gamma is considered an important immunomodulatory factor as well as a fatty acid regulator. In this study, we researched the expression of PPAR-gamma in renal ischemia-reperfusion injury of the rat. The right kidney was harvested and left renal artery and vein were clamped under laparotomy. The kidney was reperfused after 90 minutes of ischemia, and rats were sacrificed at 0, 1.5, 3, 5, 12, and 24 hours after reperfusion. PPAR-gamma expression was analyzed by immunohistochemical staining using monoclonal antibody. In normal kidney, PPAR-gamma staining was weak on endothelial cells, including mesangial cells. On the other hand, PPAR-gamma staining was weak on interstitial cells and strong on collecting ducts of medulla. From 1.5 to 5 hours after reperfusion, PPAR-gamma staining was strong on endothelial cells, moderate on interstitial cells, and strong on collecting ducts. Twelve hours after reperfusion, PPAR-gamma staining was weak on endothelial cells, moderate on interstitial cells, and strong on collecting ducts. PPAR-gamma is induced on collecting ducts, interstitial cells, and endothelial cells in a rat model having renal ischemia-reperfusion injury. |
| Databáze: | OpenAIRE |
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