Efficient inactivation of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in human apheresis platelet concentrates with amotosalen and ultraviolet A light
| Autor: | Ahmad A Almalki, Esam I. Azhar, Thamir A. Alandijany, Qossay Abunada, Marcus Picard-Maureau, Ahmed M. Hassan, Salwa Hindawi, Maha A. Badawi, Hosam M. Zowawi, Ghazi A. Damanhouri, Maiman M Bayoumi, Ahmed M. Tolah, Sherif A. El-Kafrawy |
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| Rok vydání: | 2022 |
| Předmět: |
Blood Platelets
Amotosalen Ultraviolet Rays viruses Clinical Biochemistry Pathogen reduction Réduction des agents pathogènes Furocoumarins Humans Medicine Platelet Sécurité transfusionnelle Virus quantification SARS-CoV-2 business.industry Biochemistry (medical) COVID-19 Hematology Virology In vitro Titer Apheresis Viral replication Cell culture Blood Component Removal RNA Viral Virus Inactivation Original Article business Blood safety |
| Zdroj: | Transfusion Clinique et Biologique |
| ISSN: | 1246-7820 |
| DOI: | 10.1016/j.tracli.2021.08.005 |
| Popis: | Objectives The detection of SARS-CoV-2 RNA in blood and platelet concentrates from asymptomatic donors, and the detection of viral particles on the surface and inside platelets during in vitro experiments, raised concerns over the potential risk for transfusion-transmitted-infection (TTI). The objective of this study was to assess the efficacy of the amotosalen/UVA pathogen reduction technology for SARS-CoV-2 in human platelet concentrates to mitigate such potential risk. Material and methods Five apheresis platelet units in 100% plasma were spiked with a clinical SARS-CoV-2 isolate followed by treatment with amotosalen/UVA (INTERCEPT Blood System), pre- and posttreatment samples were collected as well as untreated positive and negative controls. The infectious viral titer was assessed by plaque assay and the genomic titer by quantitative RT-PCR. To exclude the presence of infectious particles post-pathogen reduction treatment below the limit of detection, three consecutive rounds of passaging on permissive cell lines were conducted. Results SARS-CoV-2 in platelet concentrates was inactivated with amotosalen/UVA below the limit of detection with a mean log reduction of > 3.31 ± 0.23. During three consecutive rounds of passaging, no viral replication was detected. Pathogen reduction treatment also inhibited nucleic acid detection with a log reduction of > 4.46 ± 0.51 PFU equivalents. Conclusion SARS-CoV-2 was efficiently inactivated in platelet concentrates by amotosalen/UVA treatment. These results are in line with previous inactivation data for SARS-CoV-2 in plasma as well as MERS-CoV and SARS-CoV-1 in platelets and plasma, demonstrating efficient inactivation of human coronaviruses. |
| Databáze: | OpenAIRE |
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