| Autor: |
Alex, R., K.P. Ramesha, Singh, U., Kumar, S., Alyethodi, R. R., Deb, R., Sharma, S., Sengar, G. S., Ashish, Prakash, B. |
| Předmět: |
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| Zdroj: |
Scopus-Elsevier |
| Popis: |
In the antiviral host defense mechanisms, the role of mammalian OAS/RNASEL pathway is very significant. These enzymes are interferon-inducible and activated by binding to double-stranded RNA (dsRNA) which are present in virus infected cells. The OAS proteins functions through its receptor, the 2-5Adependent ribonuclease (RNaseL) and activated OAS-RNaseL system degrades viral and cellular RNA and subsequently inhibits protein synthesis. Polymorphisms in the human and equine OAS gene cluster have been previously utilized for casecontrol analysis of virus-induced disease. But no polymorphisms have yet been identified in the bovine OAS1 genes for use in similar case-control studies. The promoter and coding regions of the OAS1 gene was amplified and screened for polymorphisms by PCR-SSCP and sequencing in Sahiwal and Frieswal animals. Two SNPs have been identified in the promoter region of OAS1 gene, which have predicted to create/delete sites for transcription factors. Specific amplification of the exonic regions of the OAS1 gene have identified 26 SNPs and one dinucleotide repeats, among them 14 are mis-sense variants. These polymorphisms are the first to be reported in OAS1 gene and will facilitate future case-control studies of cattle susceptibility to infectious diseases. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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