| Autor: |
Qiang Zhang, Guannan Guan, Ruimeng Sun, Qing Chen, Demin Zhou, Xiaoyou Wang, Peiyao Wu, Shuangming Kang, Dawei Chen, Bing He, Dan Yang, Feiyang Deng, Wenbing Dai, Xueqing Wang, Hua Zhang |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Biomaterials. 251:120008 |
| ISSN: |
0142-9612 |
| DOI: |
10.1016/j.biomaterials.2020.120008 |
| Popis: |
Active targeting modification is one of the foremost nanomedicine strategies for the efficacy improvement. Compared to the homogeneous ligandation on spherical nanocarriers, non-spherical nanomedicines usually make the ligand modification more complicated. The modified ligands always exhibit anisotropy and heterogeneity. However, there is very little systematic study on these diversified anisotropic modifications. The efficacy difference and underlying mechanism were still unclear. Here, we separately fabricated hybrid nanodiscs (NDs) conjugated with cRGD on the edge and plane surfaces to engineer two anisotropic targeting nanocarriers (E-cRGD-NDs and P-cRGD-NDs, respectively) for gene delivery. The ligand anisotropy endowed NDs with diversified cellular interactions, and caused different efficacies between E-cRGD-NDs and P-cRGD-NDs. Of note, E-cRGD-NDs showed significant superiority in siRNA loading, cellular uptake, silence efficiency, protein expression and even in vivo efficacy. The mechanism investigation revealed the functional anisotropy specifically for E-cRGD-NDs. The edge modification of cRGD efficiently separated the targeting and siRNA loading domains, maximizing their respective functions. These findings reflected the unique effect of ligand anisotropy, also provided a new strategy for the targeting screening of extensive nanomedicines. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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