DegP protease is essential for tolerance to salt stress in the plant growth-promoting bacterium Gluconacetobacter diazotrophicus PAL5
| Autor: | Ana Laura Boechat, Luciano de Souza Vespoli, Vanildo Silveira, Lucas Zanchetta Passamani, Fabiano Silva Soares, Vivian Ribeiro Pimentel, Leandro Fernandes Andrade, Mariana Ramos Leandro, Gonçalo Apolinário de Souza Filho, Julia Rosa Moreira |
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| Rok vydání: | 2021 |
| Předmět: |
Iron
medicine.medical_treatment Mutant Sodium Chloride Biology Microbiology 03 medical and health sciences Bacterial Proteins medicine Heat-Shock Proteins 030304 developmental biology 0303 health sciences Protease 030306 microbiology Abiotic stress Serine Endopeptidases Gene Expression Regulation Bacterial Periplasmic space biology.organism_classification Transport protein Gluconacetobacter Biochemistry Osmoprotectant Periplasmic Proteins Bacterial outer membrane Bacteria Peptide Hydrolases |
| Zdroj: | Microbiological Research. 243:126654 |
| ISSN: | 0944-5013 |
| Popis: | The use of plant growth-promoting bacteria represents an alternative to the massive use of mineral fertilizers in agriculture. However, some abiotic stresses commonly found in the environment, like salinity, can affect the efficiency of this approach. Here, we investigated the key mechanisms involved in the response of the plant growth-promoting bacterium Gluconacetobacter diazotrophicus to salt stress by using morphological and cell viability analyses, comparative proteomics, and reverse genetics. Our results revealed that the bacteria produce filamentous cells in response to salt at 100 mM and 150 mM NaCl. However, such a response was not observed at higher concentrations, where cell viability was severely affected. Proteomic analysis showed that salt stress modulates proteins involved in several pathways, including iron uptake, outer membrane efflux, osmotic adjustment, cell division and elongation, and protein transport and quality control. Proteomic data also revealed the repression of several extracytoplasmic proteins, especially those located at periplasm and outer membrane. The role of such pathways in the tolerance to salt stress was analyzed by the use of mutant defectives for Δtbdr (iron uptake), ΔmtlK and ΔotsA (compatible solutes synthesis), and ΔdegP (quality control of nascent extracytoplasmic proteins). ΔdegP presented the highest sensitivity to salt stress, Δtbdr, andΔmtlK also showed increased sensitivity, but ΔotsA was not affected. This is the first demonstration that DegP protein, a protease with minor chaperone activity, is essential for tolerance to salt stress in G. diazotrophicus. Our data contribute to a better understanding of the molecular bases that control the bacterial response/tolerance to salt stress, shedding light on quality control of nascent extracytoplasmic proteins. |
| Databáze: | OpenAIRE |
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