Progesterone is Essential for Maintenance of Tace/Adam17 mRNA Expression, But not EGF-like Factor, in Cumulus Cells, Which Enhances the EGF Receptor Signaling Pathway During In Vitro Maturation of Porcine COCs
| Autor: | Yasuhisa Yamashita, Ikko Kawashima, Masayuki Shimada, Yosuke Gunji, Mitsugu Hishinuma |
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| Rok vydání: | 2010 |
| Předmět: |
endocrine system
Time Factors Swine ADAM17 Protein Biology Epiregulin Amphiregulin Progesterone receptor medicine Animals Receptor Progesterone reproductive and urinary physiology DNA Primers Cumulus Cells Epidermal Growth Factor Reverse Transcriptase Polymerase Chain Reaction urogenital system Luteinizing Hormone Oocyte Molecular biology In vitro Cell biology In vitro maturation ADAM Proteins Mifepristone medicine.anatomical_structure Gene Expression Regulation Oocytes Phosphorylation Animal Science and Zoology Follicle Stimulating Hormone hormones hormone substitutes and hormone antagonists Signal Transduction |
| Zdroj: | Journal of Reproduction and Development. 56:315-323 |
| ISSN: | 1348-4400 0916-8818 |
| DOI: | 10.1262/jrd.09-199h |
| Popis: | During in vitro maturation of porcine cumulus-oocyte complexes (COCs), progesterone was secreted from cumulus cells and acted on the cumulus cells themselves, which required for cumulus expansion and oocyte maturation. EGF-like factor (amphiregulin, AREG; epiregulin, EREG) and its protease, TACE/ADAM17, are also expressed in cumulus cells, and thereby, soluble EGF domain was acted on the EGF receptor expressed on cumulus cells. In this study, we examined the relationship between progesterone function and EGF-like factor stimuli in cumulus cells of porcine COCs. When COCs were cultured with FSH and LH, Areg, Ereg and Tace/Adam17 were expressed in cumulus cells. Treatment with a progesterone receptor (PGR) antagonist, RU486, did not affect the Areg and Ereg mRNA expression levels at any culture time points. However, the Tace/Adam17 mRNA level, protein level and its activity were significantly suppressed by RU486 at the 30 or 40 h time point. At 20 h of culture, phosphorylation of ERK1/2 and the expressions of target genes (Has2, Tnfaip6 and Ptgs2) were not suppressed by RU486; however, at 40 h, ERK1/2 phosphorylation and the target gene expression levels were significantly downregulated by RU486 in cumulus cells. Furthermore, the negative effects of RU486 at 40 h were overcome by the addition of EGF. These results indicated that the level of TACE/ADAM17 in cumulus cells was regulated by the progesterone-PGR pathway during in vitro maturation of porcine COCs. Therefore, we concluded that the progesterone-induced TACE/ADAM17 leads to production of soluble EGF domain from cumulus cells, which enhances functional changes of cumulus cells and progresses meiotic maturation of oocytes during in vitro maturation of porcine COCs. |
| Databáze: | OpenAIRE |
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