Calmodulin-like protein increases filopodia-dependent cell motility via up-regulation of myosin-10
| Autor: | Amy S. Mauer, Emanuel E. Strehler, Richard D. Bennett |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Cellular differentiation
Molecular Sequence Data Motility Biology Myosins Transfection Biochemistry Calmodulin Cell Movement Myosin Humans Amino Acid Sequence Pseudopodia Molecular Biology DNA Primers Base Sequence Reverse Transcriptase Polymerase Chain Reaction Cell migration Cell Biology Molecular biology Peptide Fragments Cell biology Cell culture Wound healing Filopodia Cell Division HeLa Cells |
| Zdroj: | The Journal of biological chemistry. 282(5) |
| ISSN: | 0021-9258 |
| Popis: | Human calmodulin-like protein (CLP) is an epithelial-specific protein that is expressed during cell differentiation but down-regulated in primary cancers and transformed cell lines. Using stably transfected and inducible HeLa cell lines, we found that CLP expression did not alter the proliferation rate and colony-forming potential of these cells. However, remarkable phenotypic changes were observed in CLP-expressing compared with control cells. Soft agar colonies of CLP-expressing cells had rough boundaries, with peripheral cells migrating away from the colony. Cells expressing CLP displayed a striking increase in the number and length of myosin-10-positive filopodia and showed increased mobility in a wound healing assay. This increase in wound healing capacity was prevented by small interference RNA-mediated down-regulation of myosin-10. Fluorescence microscopy and Western blotting revealed that CLP expression results in up-regulation of its target protein, myosin-10. This up-regulation occurs at the protein level by stabilization of myosin-10. Thus, CLP functions by increasing the stability of myosin-10, leading to enhanced myosin-10 function and a subsequent increase in filopodial dynamics and cell migration. In stratified epithelia, CLP may be required during terminal differentiation to increase myosin-10 function as cells migrate toward the upper layers and establish new adhesive contacts. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|