Hydrogenated diglucose detergents for membrane-protein extraction and stabilization

Autor: Marine Soulié, Carolyn Vargas, Pierre Guillet, Anass Jawhari, Kelly Garnier, Grégory Durand, Gildas Nyame Mendendy Boussambe, Florian Mahler, Sébastien Igonet, Christine Ebel, Sandro Keller
Přispěvatelé: Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), CHEM2STAB, Avignon Université (AU), Molecular Biophysics, Technische Universität Kaiserslautern (TU Kaiserslautern), CALIXAR, Institut de biologie structurale (IBS - UMR 5075 ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), University of Kaiserslautern [Kaiserslautern], KE 1478/7 1, Deutsche Forschungsgemeinschaft, ANR-14-LAB7-0002, Agence Nationale de la Recherche, ANR-14-LAB7-0002,CHEM2STAB,Nouvelles Approches Moléculaires pour l'isolement de Protéines Membranaires(2014), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Langmuir
Langmuir, 2019, 35 (12), pp.4287-4295. ⟨10.1021/acs.langmuir.8b02842⟩
Langmuir, American Chemical Society, 2019, 35 (12), pp.4287-4295. ⟨10.1021/acs.langmuir.8b02842⟩
ISSN: 0743-7463
1520-5827
DOI: 10.1021/acs.langmuir.8b02842⟩
Popis: International audience; We report herein the design and synthesis of a novel series of alkyl glycosides detergents consisting of a nonionic polar headgroup that comprises two glucose moieties in a branched arrangement (DG), onto which octane-, decane- and dodecanethiols were grafted leading to ODG, DDG, and DDDG detergents. Micellization in aqueous solution was studied by isothermal titration calorimetry (ITC), 1H NMR spectroscopy, and surface tensiometry (SFT). Critical micellar concentration values were found to decrease by a factor of ~10 for each pair of methylene groups added to the alkyl chain, ranging from ~0.05 mM to 9 mM for DDDG and ODG, respectively. Dynamic light scattering (DLS) and analytical ultracentrifugation sedimentation velocity (AUC-SV) experiments were used to investigate the size and the composition of the micellar aggregates, showing that the aggregation number significantly increased from ~40 for ODG to ~80 for DDDG. All new compounds were able to solubilize MPs from bacterial membranes, insect cells, as well as Madin Darby Canine Kidney (MDCK) cells. In particular, native human adenosine receptor (A2AR) and bacterial transporter BmrA were solubilized efficiently. A striking thermostability improvement of +13 and +9°C when ODG and DDG were respectively applied to wild type and full length A2AR. Taken together, this novel detergent series shows promising detergent potency for solubilization and stabilization of MPs and, thus, makes a valuable addition to the chemical toolbox available for extracting and handling these important but challenging membrane protein targets.
Databáze: OpenAIRE
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