Use of a rat ex-vivo testis culture method to assess toxicity of select known male reproductive toxicants
| Autor: | Thomas K. Baker, Marie-Hélène Perrard, Keith M. Goldstein, Seyler David Edward, Philippe Durand |
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| Přispěvatelé: | Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut de Génomique Fonctionnelle de Lyon (IGFL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine endocrine system medicine.medical_specialty Pilot Projects Acetates Biology Toxicology Rats Sprague-Dawley Tissue Culture Techniques Andrology 03 medical and health sciences Phenols In vivo Internal medicine Toxicity Tests medicine Animals Benzhydryl Compounds Cells Cultured ComputingMilieux_MISCELLANEOUS Sertoli Cells urogenital system Meiosis II [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology Seminiferous Tubules Sertoli cell In vitro Rats 3. Good health Dinitrobenzenes Germ Cells 030104 developmental biology Seminiferous tubule medicine.anatomical_structure Endocrinology [SDV.TOX]Life Sciences [q-bio]/Toxicology Toxicity Spermatogenesis Hexachlorocyclohexane Ex vivo |
| Zdroj: | Reproductive Toxicology Reproductive Toxicology, Elsevier, 2016, 60, pp.92-103. ⟨10.1016/j.reprotox.2016.01.003⟩ Reproductive Toxicology, 2016, 60, pp.92-103. ⟨10.1016/j.reprotox.2016.01.003⟩ |
| ISSN: | 0890-6238 |
| Popis: | Due to the complex physiology of the testes, in vitro models have been largely unsuccessful at modeling testicular toxicity in vivo. We conducted a pilot study to evaluate the utility of the Durand ex vivo rat seminiferous tubule culture model [1-3] that supports spermatogenesis through meiosis II, including the formation of round spermatids. We used this system to evaluate the toxicity of four known testicular toxicants: 1,3-dinitrobenzene (DNB), 2-methoxyacetic acid (MAA), bisphenol A (BPA), and lindane over 21 days of culture. This organotypic culture system demonstrated the ability to successfully model in vivo testicular toxicity (Sertoli cell toxicity and disruption of meiosis) for all four compounds. These findings support the application of this system to study molecules and evaluate mechanisms of testicular toxicity. |
| Databáze: | OpenAIRE |
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