Hydroxytyrosol inhibits MAO isoforms and prevents neurotoxicity inducible by MPP invivo
| Autor: | Sergio Montes, Moisés Rubio-Osornio, José Guillermo Avila-Acevedo, Gabriela Pérez-Barrón, Antonio Monroy-Noyola, Sara García-Jiménez, Luis C Rios |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
1-Methyl-4-phenylpyridinium Monoamine Oxidase Inhibitors Monoamine oxidase Dopamine Pharmacology Neuroprotection Antioxidants General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Catecholamines 0302 clinical medicine medicine Animals Protein Isoforms Rats Wistar Monoamine Oxidase chemistry.chemical_classification Reactive oxygen species General Immunology and Microbiology Dopaminergic Neurotoxicity Parkinson Disease Phenylethyl Alcohol medicine.disease Corpus Striatum Rats Apomorphine 030104 developmental biology chemistry 030220 oncology & carcinogenesis Hydroxytyrosol medicine.drug |
| Zdroj: | Frontiers in Bioscience. 12:25-37 |
| ISSN: | 1945-0524 1945-0516 |
| DOI: | 10.2741/s538 |
| Popis: | Parkinson's disease is considered to be due to an increase in the catabolism of dopamine by the action of monoamine oxidase (MAO) enzymes which leads to an increase in reactive oxygen species (ROS) and loss of dopaminergic neurons. Here, in a model of neurotoxicity inducible by 1-methyl-4-phenylpyridinium (MPP+), we tested the effect of hydroxytyrosol (HTy), a potent antioxidant, on generation of ROS. Five minutes after a single intravenous administration of 1.5 mg/Kg of Hty, Wistar rats received an intrastriatal micro-injection of 10 micrograms of MPP+ while control animals received saline solution. Six days later, all animals were treated with apomorphine (1 mg/Kg), subcutaneously and ipsilateral rotations were assessed within an hour. Then, the rats were sacrificed, striatal tissues were removed and their catecholamines and MAO-A and B activities were quantitated. Pretreatment with HTy significantly diminished the number of ipsilateral rotations. This recovery correlated with significant preservation of striatal dopamine and significant inhibition of of the MAO activity. These results are consistent with the inhibitory effect of HTy on the MAO isoforms and form a basis for the neuroprotective mechanism of this phenylpropanoid in MPP+ induced Parkinson's disease. |
| Databáze: | OpenAIRE |
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