The Role of CD44-Hyaluronic Acid Interaction in Exogenous Mesenchymal Stem Cells Homing to Rat Remnant Kidney
| Autor: | Li Ma, Jia-Ning Miao, Yu Bai, Xiaohui Bian, Liqiang Zheng, Qiu-Ling Fan, Ping He, Xuesong Su, Jianfei Ma, Xu Yang, Li-ning Wang, Feng Du, Jiangmin Feng, Guangyu Zhou, Detian Li, Guangping Sun, Yanqiu Wang, Wei Guo, Na Liu, Guifeng Zhao |
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| Rok vydání: | 2013 |
| Předmět: |
Male
lcsh:Diseases of the circulatory (Cardiovascular) system Pathology medicine.medical_specialty Kidney Cortex lcsh:RC870-923 Kidney Mesenchymal Stem Cell Transplantation Nephrectomy Flow cytometry Rats Sprague-Dawley Masson's trichrome stain chemistry.chemical_compound 5/6 Nephrectomy Cell Movement Hyaluronic acid Chronic renal failure lcsh:Dermatology medicine Animals Urea CD44 Hyaluronic Acid Mesenchymal stem cell biology medicine.diagnostic_test General Medicine lcsh:RL1-803 lcsh:Diseases of the genitourinary system. Urology Rats Hyaluronan Receptors medicine.anatomical_structure chemistry lcsh:RC666-701 Nephrology Creatinine biology.protein Immunohistochemistry Kidney Diseases Cardiology and Cardiovascular Medicine Homing (hematopoietic) |
| Zdroj: | Kidney & Blood Pressure Research, Vol 38, Iss 1, Pp 11-20 (2014) |
| ISSN: | 1423-0143 1420-4096 |
| DOI: | 10.1159/000355749 |
| Popis: | Background/Aims: The aim of our study was to reveal the role of CD44-Hyaluronic acid (HA) in the homing and improving renal function of systemically transplanted MSCs in chronic renal failure. Methods: First, a remnant kidney model was established in rats and the expression of HA was determined using immunohistochemistry (IHC) and western blotting. Next, chemotaxis assay using flow cytometry, and cell migration assay of MSCs were performed in vitro. Then, MSCs were transplanted into rats, thus, sprague-Dawley (SD) rats were randomly divided into sham group, 5/6 nephrectomy (5/6 Nx) group, MSC group and MSC/Anti-CD44 group (n = 8 for all groups). Migration of MSCs to the kidney in these rats was assessed by using cell tracking experiments, and tissue damage was evaluated by morphological analysis using Masson's trichrome staining and periodic acid Schiff staining. Results: HA was significantly observed in 5/6 Nx group, but not in sham group. Meanwhile, HA was discovered induced MSCs migration remarkably (p < 0.05) and anti-CD44 antibody inhibited the migration significantly (p < 0.05) in vitro. In vivo, the GFP-MSCs were observed in MSC group and the cells reduced in MSC/Anti-CD44 groups, especially, in the tubulointerstitium. Conclusion: Our findings reveal that CD44-HA has the potential to induce MSCs homing to injured tissue, while its effect on the ability of MSCs, improving tissue function, is not significant. |
| Databáze: | OpenAIRE |
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