Mouse Middle Ear Ion Homeostasis Channels and Intercellular Junctions
| Autor: | Jacqueline M. DeGagne, Lisa M. Morris, Frances A. Hausman, Dennis R. Trune, J. Beth Kempton |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Pathology
Anatomy and Physiology Mouse lcsh:Medicine Otology Ear Infections Cell junction Pediatrics Ion Channels Mice 0302 clinical medicine Immune Physiology Homeostasis lcsh:Science Hearing Disorders 0303 health sciences Mice Inbred BALB C Multidisciplinary Cell Surface Molecules Animal Models Immunohistochemistry Sensory Systems Cell biology medicine.anatomical_structure Intercellular Junctions Middle ear Medicine Research Article medicine.medical_specialty Cell Physiology Ear Middle Biology 03 medical and health sciences Model Organisms medicine otorhinolaryngologic diseases Animals Inner ear Ion channel 030304 developmental biology Pediatric Otorhinolaryngology lcsh:R Epithelium Otitis Media Ion homeostasis Otorhinolaryngology Organ of Corti lcsh:Q sense organs Fluid Physiology 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 6, p e39004 (2012) |
| ISSN: | 1932-6203 |
| Popis: | Hypothesis The middle ear contains homeostatic mechanisms that control the movement of ions and fluids similar to those present in the inner ear, and are altered during inflammation. Background The normal middle ear cavity is fluid-free and air-filled to allow for effective sound transmission. Within the inner ear, the regulation of fluid and ion movement is essential for normal auditory and vestibular function. The same ion and fluid channels active in the inner ear may have similar roles with fluid regulation in the middle ear. Methods Middle and inner ears from BALB/c mice were processed for immunohistochemistry of 10 specific ion homeostasis factors to determine if similar transport and barrier mechanisms are present in the tympanic cavity. Examination also was made of BALB/c mice middle ears after transtympanic injection with heat-killed Haemophilus influenza to determine if these channels are impacted by inflammation. Results The most prominent ion channels in the middle ear included aquaporins 1, 4 and 5, claudin 3, ENaC and Na+,K+-ATPase. Moderate staining was found for GJB2, KCNJ10 and KCNQ1. The inflamed middle ear epithelium showed increased staining due to expected cellular hypertrophy. Localization of ion channels was preserved within the inflamed middle ear epithelium. Conclusions The middle ear epithelium is a dynamic environment with intrinsic mechanisms for the control of ion and water transport to keep the middle ear clear of fluids. Compromise of these processes during middle ear disease may underlie the accumulation of effusions and suggests they may be a therapeutic target for effusion control. |
| Databáze: | OpenAIRE |
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