Isolation, structure determination, and antiaging effects of 2,3-pentanediol from endophytic fungus of Curcuma amada and docking studies
| Autor: | Shailendra K. Gupta, Arvind S. Negi, Sudeep Tiwari, Shilpi K. Saikia, Rakesh Pandey, Suchitra Banerjee, Pallavi Pandey, Sailendra Singh |
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| Rok vydání: | 2014 |
| Předmět: |
Aging
Antioxidant medicine.medical_treatment Molecular Sequence Data Microbial Sensitivity Tests Plant Science Biology Antioxidants Plant use of endophytic fungi in defense Glycols Curcuma food Fusarium Fusarium oxysporum Botany RNA Ribosomal 18S medicine Animals Caenorhabditis elegans Base Sequence Sequence Analysis RNA Cell Biology General Medicine Antimicrobial biology.organism_classification food.food Anti-Bacterial Agents Rhizome Molecular Docking Simulation Biochemistry Docking (molecular) Zingiberaceae Curcuma amada |
| Zdroj: | Protoplasma. 251:1089-1098 |
| ISSN: | 1615-6102 0033-183X |
| Popis: | An endophytic fungus was isolated from the rhizomes of Curcuma amada (Zingiberaceae), which was identified as Fusarium oxysporum on the basis of its morphological and molecular characters. Chromatographic separation and spectroscopic analysis of the fungal metabolite (chloroform extract) led to the identification of one pure compound having molecular formula C5H12O2, i.e., 2,3-pentanediol (1). Activity analysis of compound 1 demonstrated improved antiaging (antioxidant, thermotolerance) properties against Caenorhabditis elegans, in comparison to a similar, commercially available molecule i.e., 1,5-pentanediol (2). The effective (lower) concentration of 1 significantly showed (28.6 %) higher survival percentage of the worms under thermal stress (37 oC) compared to its higher concentration (25.3 %), while similar trends were followed in oxidative stress where (22.2 %) higher survival percentage was recorded in comparison to untreated control. The compound 1, however, lacked potential antimicrobial activity, indicating the plausible ramification of the position of OH group in such bioactive molecules. In silico evaluation of these molecules against common as well as unique targets corroborated better antiaging potential of 1 in comparison to that of 2. The results for the first time indicated that the utilization of the endophytic fungi of C. amada could, thus, be a possible source for obtaining non-plant-based bioactive compounds having broader therapeutic applications pertaining to age-related progressions. |
| Databáze: | OpenAIRE |
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