Polyphenolic compounds from flowers of Hibiscus rosa-sinensis Linn. and their inhibitory effect on alkaline phosphatase enzyme activity in vitro
Autor: | Enas N. Daniel, Iman B. Shaheed, Sally M. Abdel-Latif, Josline Y. Salib, Mohamed S. Hifnawy, Shadia M. Azzam |
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Rok vydání: | 2011 |
Předmět: |
Chromatography
Magnetic Resonance Spectroscopy biology Chemistry Plant Extracts Ultraviolet Rays Chemical structure Hibiscus rosa-sinensis Polyphenols Flowers In Vitro Techniques biology.organism_classification Hibiscus Alkaline Phosphatase General Biochemistry Genetics and Molecular Biology Enzyme assay In vitro Phytochemical Polyphenol Botany biology.protein Alkaline phosphatase Enzyme Inhibitors |
Zdroj: | Zeitschrift fur Naturforschung. C, Journal of biosciences. 66(9-10) |
ISSN: | 0939-5075 |
Popis: | Graded concentrations (0.1 - 100 mg/mL reaction mixture) of the methanolic extract of the flowers of Hibiscus rosa-sinensis Linn., its water-soluble fraction as well as compounds isolated from this fraction were tested for their inhibitory effect on alkaline phosphatase enzyme activity in vitro. Both the methanolic extract and its water-soluble fraction showed significant inhibitory effects on the enzyme activity in vitro. On screening the activity of the compounds isolated from the water-soluble fraction, its high inhibitory activity was attributed to the presence of quercetin-7-O-galactoside which showed a high potent inhibition of the enzyme activity reaching 100% at 100 mg/mL reaction mixture. Phytochemical investigations of the water-soluble fraction were also carried out and afforded ten polyphenolic compounds including two new natural compounds, namely kaempferol- 7-O-[6´´´-O-p-hydroxybenzoyl-β-D-glucosyl-(1≙6)-β-D-glucopyranoside] and scutellarein-6- O-α-L-rhamnopyranoside-8-C-β-D-glucopyranoside). The chemical structure of the isolated compounds was elucidated on the basis of chemical and spectral data. |
Databáze: | OpenAIRE |
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