Polyphenols protect against age-associated apoptosis in female rat cochleae
Autor: | Juan Riestra-Ayora, Ricardo Sanz-Fernández, Esperanza Cuadrado, Carolina Sánchez-Rodríguez |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Aging medicine.medical_specialty Cell Envejecimiento Presbycusis Apoptosis Antioxidantes medicine.disease_cause Antioxidants Rats Sprague-Dawley 03 medical and health sciences Adenosine Triphosphate 0302 clinical medicine Annexin Internal medicine Gene expression medicine Animals Humans Annexin A5 Caspase Cochlea biology Caspase 3 Chemistry Deoxyguanosine Polyphenols food and beverages medicine.disease Rats Disease Models Animal Oxidative Stress 030104 developmental biology medicine.anatomical_structure Endocrinology 8-Hydroxy-2'-Deoxyguanosine Nutrición biology.protein Female Tumor Suppressor Protein p53 Geriatrics and Gerontology Gerontology 030217 neurology & neurosurgery Oxidative stress Signal Transduction |
Zdroj: | Biogerontology. 19:159-169 |
ISSN: | 1573-6768 1389-5729 |
Popis: | Dietary antioxidants, polyphenols, have been found to be beneficial in protecting against the generation of oxidative stress in various diseases associated with aging. Age-related hearing loss (AHL) is the number one neurodegenerative disorder on our aged population. Sprague–Dawley rats divided into five groups according to their age (3, 6, 12, 18 and 24 months old) and treated with 100 mg/day/kg body weight of polyphenols were used. Then, cochleae were harvested to measure caspase activities (- 3, - 8 and - 9), caspase-3 gene expression, ATP levels, Bax, BcL-2 and p53 levels. 8-OHdG levels (marker of DNA oxidative damage) and annexin-V were also measured in cochleae. Increased levels of caspase-3 and 9 in cochlea were observed with age and this effect was attenuated by polyphenol treatment. In addition, ATP and Bcl-2 levels in older rats were recovered after administration of polyphenols, while Bax and p53 levels protein decreased. Oral supplementation with polyphenols also reduces DNA oxidative damage of cochlear cell. Treatment with polyphenols inhibits the activation of age-related apoptotic signaling by decreasing oxidative stress inside the rat cochlea. Spanish Fondo de Investigaciones Sanitarias FIS PI09/02472 European University of Madrid 3.805 JCR (2018) Q2, 15/53 Geriatrics & Gerontology 1.301 SJR (2018) Q1, 3/43 Gerontology, 22/114 Geriatrics and Gerontology; Q2, 15/36 Aging No data IDR 2018 UEM |
Databáze: | OpenAIRE |
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