Effects of low-dose irradiation on mice with Escherichia coli -induced sepsis
| Autor: | Seon Young Nam, Kyung Hee Song, Jie-Young Song, Seung Youn Jung, Hunjoo Ha, Sang-Gu Hwang, Seong Ho Kho |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Colony Count Microbial Inflammation Spleen Biology Kidney Nitric Oxide Toxicology Microbiology Nitric oxide Proinflammatory cytokine Sepsis Mice 03 medical and health sciences chemistry.chemical_compound Immune system Escherichia coli medicine Animals Escherichia coli Infections Escherichia coli infection Pharmacology medicine.disease Mice Inbred C57BL RAW 264.7 Cells 030104 developmental biology medicine.anatomical_structure chemistry Immunology Macrophages Peritoneal Cytokines Female medicine.symptom Whole-Body Irradiation |
| Zdroj: | Toxicology and Applied Pharmacology. 333:17-25 |
| ISSN: | 0041-008X |
| DOI: | 10.1016/j.taap.2017.08.008 |
| Popis: | Although favorable immune responses to low-dose irradiation (LDI) have been observed in normal mice, i.e., a hormesis effect, little is known about the effects of LDI in infectious diseases. In this study, we examined the effects of LDI on mice with sepsis, a severe and often lethal hyperinflammatory response to bacteria. Female C57BL/6 mice were whole-body irradiated with 10cGy 48h before Escherichia coli infection, and survival, bacterial clearance, cytokines, and antioxidants were quantified. LDI pretreatment significantly increased survival from 46.7% in control mice to 75% in mice with sepsis. The bacterial burden was significantly lower in the blood, spleen, and kidney of LDI-treated mice than in those of control septic mice. The levels of pro-inflammatory cytokines, e.g., IL-1β and IL-6, as well as anti-inflammatory IL-10 were markedly reduced in pre-LDI septic mice. Nitric oxide production by peritoneal macrophages was also reduced in pre-LDI septic mice. Immune cells in the spleen increased and Nrf2 and HO-1 were induced in pre-LDI septic mice. LDI stimulates the immune response and minimizes lethality in septic mice via enhanced bacterial clearance and reduced initial proinflammatory responses. |
| Databáze: | OpenAIRE |
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