Next-Generation Sequencing Reveals Downregulation of the Wnt Signaling Pathway in Human Dysmature Cumulus Cells as a Hallmark for Evaluating Oocyte Quality
| Autor: | Kenji Miyado, Hidekazu Saito, Woojin Kang, Ryosuke Akino, Akihiko Sekizawa, Ryouka Kawahara-Miki, Mitsuyoshi Amita, Shuji Takada, Daisuke Matsui, Eri Ishida, Takakazu Saito, Tomohiro Kono, Kuniko Tatsumi |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
endocrine system female infertility cumulus cells Ocean Engineering Biology Wnt signaling pathway 03 medical and health sciences 0302 clinical medicine Human fertilization Downregulation and upregulation Gene expression medicine Gene reproductive and urinary physiology Water Science and Technology urogenital system Embryogenesis Geology Oocyte Cell biology 030104 developmental biology medicine.anatomical_structure Apoptosis 030220 oncology & carcinogenesis next-generation sequencing oocyte quality hormones hormone substitutes and hormone antagonists |
| Zdroj: | Reproductive Medicine Volume 1 Issue 3 Pages 16-215 |
| ISSN: | 2673-3897 |
| DOI: | 10.3390/reprodmed1030016 |
| Popis: | Background: Dysmature cumulus cells are lower fertilization rates and abnormalities in embryonic development compared to maturation cumulus cells. Morphological evaluation of cumulus&ndash oocyte complexes (COCs) considered the possibility that differences may also be found in gene expression. Purpose: To identify hallmarks for evaluating oocyte quality by investigating gene expression patterns in human cumulus cells surrounding oocytes. Methods: Cumulus cells were obtained from the cumulus&ndash oocyte complex of infertile women treated with assisted reproductive technology. Based on maturity level, the cumulus cells were classified into two categories, i.e., dysmature cumulus cell (DCC) and maturation cumulus cell. DCCs were subjected to gene expression analysis using next-generation sequencing and compared with COCs that are in the process of maturation as controls. Results: The expression levels of genes involved in the Wnt signal/&beta catenin pathway were significantly reduced in DCCs compared with those in control cells. Moreover, the expression levels of genes involved in multiple pathways associated with apoptosis were also significantly reduced compared with those in control cells. Conclusions: DCCs showed significant decreases in apoptosis- and Wnt/&beta catenin signaling-associated gene expression. DCCs could be classified by morphological evaluation, and the method described in this study may be useful as an oocyte quality estimation tool. |
| Databáze: | OpenAIRE |
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