Endothelium-Derived Inhibitors Efficiently Attenuate the Aggregation and Adhesion Responses of Refrigerated Platelets
| Autor: | M. Adam Meledeo, Armando C Rodriguez, Anand K. Ramasubramanian, Robbie K. Montgomery, Andrew P. Cap, Kristin M. Reddoch, Heather F. Pidcoke |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Blood Platelets
Platelet Aggregation Cold storage Prostacyclin 030204 cardiovascular system & hematology Pharmacology Nitric Oxide Critical Care and Intensive Care Medicine Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Refrigeration Humans Medicine Platelet Platelet activation Hemostatic function biology business.industry Factor V Platelet Activation Epoprostenol chemistry Blood Preservation Immunology Emergency Medicine biology.protein business Platelet Aggregation Inhibitors Intracellular 030215 immunology medicine.drug |
| Zdroj: | Shock. 45:220-227 |
| ISSN: | 1073-2322 |
| DOI: | 10.1097/shk.0000000000000493 |
| Popis: | Refrigeration of platelets (4°C) provides the possibility of improving transfusion practice over the current standard-of-care, room temperature (RT) storage. However, the increased level of platelet activation observed at 4°C in vitro is cause for concern of uncontrolled thrombosis in vivo. In this study, we assessed the safety of 4°C-stored platelets by evaluating their response to physiologic inhibitors prostacyclin (PGI2) and nitric oxide (NO). Apheresis platelets were collected from healthy donors (n = 4) and tested on Day 1 (fresh) or Day 5 (RT- and 4°C-stored) after treatment with PGI2 and NO or not for: thrombin generation; factor V (FV) activity; intracellular free calcium, cAMP and cGMP; ATP release; TRAP-induced activation; aggregation to ADP, collagen, and TRAP, and adhesion to collagen under arterial flow. Data were analyzed using two-way ANOVA and post-hoc Tukey test for multiple comparisons, with significance set at P |
| Databáze: | OpenAIRE |
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