Continuous monitoring of interstitial tissue oxygen using subcutaneous oxygen microsensors: In vivo characterization in healthy volunteers
| Autor: | Natalie Wisniewski, Stephen C. Kanick, Peter Schneider, Kerstin Rebrin, Bruce Klitzman |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male 0301 basic medicine Transducers chemistry.chemical_element Biosensing Techniques 030204 cardiovascular system & hematology Biochemistry Oxygen Vascular occlusion Article Microcirculation Upper Extremity 03 medical and health sciences Subcutaneous Tissue 0302 clinical medicine Interstitial space Predictive Value of Tests Occlusion medicine Humans Aged Miniaturization integumentary system Reproducibility of Results Arteries Equipment Design Cell Biology Middle Aged Healthy Volunteers Oxygen tension 030104 developmental biology medicine.anatomical_structure chemistry Regional Blood Flow Feasibility Studies Female medicine.symptom Cardiology and Cardiovascular Medicine Blood Gas Monitoring Transcutaneous Perfusion Biomedical engineering Subcutaneous tissue |
| Zdroj: | Microvasc Res |
| ISSN: | 0026-2862 |
| DOI: | 10.1016/j.mvr.2019.02.002 |
| Popis: | Measurements of regional tissue oxygen serve as a proxy to monitor local perfusion and have the potential to guide therapeutic decisions in multiple clinical disciplines. Transcutaneous oximetry (tcpO(2)) is a commercially available noninvasive technique that uses an electrode to warm underlying skin tissue and measure the resulting oxygen tension at the skin surface. A novel approach is to directly measure interstitial tissue oxygen using subcutaneous oxygen microsensors composed of a biocompatible hydrogel carrier platform with embedded oxygen sensing molecules. After initial injection of the hydrogel into subcutaneous tissue, noninvasive optical measurements of phosphorescence-based emissions at the skin surface are used to sense oxygen in the subcutaneous interstitial space. The object of the present study was to characterize the in vivo performance of subcutaneous microsensors and compare with transcutaneous oximetry (tcpO(2)). Vascular occlusion tests were performed on the arms of 7 healthy volunteers, with repeated tests occurring 1 to 10 weeks after sensor injection, yielding 95 total tests for analysis. Comparative analysis characterized the response of both devices to decreases in tissue oxygen during occlusion and to increases in tissue oxygen following release of the occlusion. Results indicated: (I) time traces returned by microsensors and tcpO(2) were highly correlated, with the median (interquartile range) correlation coefficient of r=0.93 (0.10); (II) both microsensors and tcpO(2) sensed a statistically significant decrease in normalized oxygen during occlusion (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect