Stability of matrix metalloproteinase-9 as biological marker in colorectal cancer
| Autor: | Marie-Lois Ivarsson, Andreas Jonsson, Claes Hjalmarsson, Peter Falk |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research medicine.medical_specialty Colorectal cancer Matrix metalloproteinase Gastroenterology Cryopreservation 03 medical and health sciences 0302 clinical medicine Prognostic marker Intestinal mucosa Internal medicine Enzyme Stability medicine Biomarkers Tumor Humans Cervical cancer Original Paper Hematology business.industry Cancer Matrix metalloproteinase 9 General Medicine medicine.disease 030104 developmental biology Oncology Matrix Metalloproteinase 9 030220 oncology & carcinogenesis MMPs business Colorectal Neoplasms MMP-9 |
| Zdroj: | Medical Oncology (Northwood, London, England) |
| ISSN: | 1559-131X |
| Popis: | Matrix metalloproteinases (MMPs) are believed to be of importance in the growth and spread of colorectal cancer (CRC). MMP-9 level has been suggested as a biological predictor of prognosis in CRC as well as in other types of cancer such as breast and cervical cancer. The purpose of this study was to investigate the stability over time of MMP-9 in cryopreserved plasma, colorectal tumor tissue extract and macroscopically tumor-free colon mucosa tissue extract samples. Plasma and tissue samples were taken from patients at primary CRC surgery and analyzed for MMP-9. Aliquots of samples from the same patients were stored at – 80 °C pending analysis. These aliquots were analyzed using identical methods after storage periods of nine (plasma) and twelve (tissue) years. No significant difference in plasma MMP-9 concentration was seen between baseline samples and those after 9 years of cryopreservation (median values 9.9 and 9.7 ng/mL, respectively; p > 0.05). MMP-9 levels in the tumor-free tissue extracts had increased to baseline (median values 7.1 and 8.1 ng/mL, respectively; p |
| Databáze: | OpenAIRE |
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