A 12-residue epitope displayed on phage T7 reacts strongly with antibodies against foot-and-mouth disease virus

Autor:	Chin Chin Sieo, Chuan Loo Wong, Wen Siang Tan, Amir Syahir, Chean Yeah Yong, Azira Muhamad, Abdul Rahman Omar
Rok vydání:	2018
Předmět:	0301 basic medicine Serotype Antigenicity Phage display 040301 veterinary sciences viruses Antibodies Viral Applied Microbiology and Biotechnology Epitope Virus 0403 veterinary science Epitopes 03 medical and health sciences Bacteriophage T7 Animals Coding region biology 04 agricultural and veterinary sciences General Medicine biology.organism_classification Virology 030104 developmental biology Foot-and-Mouth Disease Virus Foot-and-Mouth Disease biology.protein Capsid Proteins Cattle Foot-and-mouth disease virus Antibody Biotechnology
Zdroj:	Applied Microbiology and Biotechnology. 102:4131-4142
ISSN:	1432-0614 0175-7598
DOI:	10.1007/s00253-018-8921-9
Popis:	Foot-and-mouth disease (FMD) is a major threat to the livestock industry worldwide. Despite constant surveillance and effective vaccination, the perpetual mutations of the foot-and-mouth disease virus (FMDV) pose a huge challenge to FMD diagnosis. The immunodominant region of the FMDV VP1 protein (residues 131–170) displayed on phage T7 has been used to detect anti-FMDV in bovine sera. In the present study, the functional epitope was further delineated using amino acid sequence alignment, homology modelling and phage display. Two highly conserved regions (VP1145–152 and VP1159–170) were identified among different FMDV serotypes. The coding regions of these two epitopes were fused separately to the T7 genome and displayed on the phage particles. Interestingly, chimeric phage displaying the VP1159–170 epitope demonstrated a higher antigenicity than that displaying the VP1131–170 epitope. By contrast, phage T7 displaying the VP1145–152 epitope did not react significantly with the anti-FMDV antibodies in vaccinated bovine sera. This study has successfully identified a smaller functional epitope, VP1159–170, located at the C-terminal end of the structural VP1 protein. The phage T7 displaying this shorter epitope is a promising diagnostic reagent to detect anti-FMDV antibodies in vaccinated animals.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56f3c818fccbe64f43bc36d66f1a9db5 https://doi.org/10.1007/s00253-018-8921-9 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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