Comparative Study of IS 6110 Restriction Fragment Length Polymorphism and Variable-Number Tandem-Repeat Typing of Mycobacterium tuberculosis Isolates in the Netherlands, Based on a 5-Year Nationwide Survey
| Autor: | Connie Erkens, Kristin Kremer, Philip Supply, Gerard de Vries, Jakko van Ingen, Anne-Marie van den Brandt, Arnout Mulder, M. M. G. G. Sebek, Rosa Sloot, Mimount Enaimi, Dick van Soolingen, Jessica L. de Beer |
|---|---|
| Přispěvatelé: | Graduate School, Epidemiology and Data Science |
| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Minisatellite Repeat Minisatellite Repeats Mycobacterium tuberculosis Poverty-related infectious diseases Infection and autoimmunity [N4i 3] Polymorphism (computer science) Cluster Analysis Humans Tuberculosis Typing Netherlands Genetics Molecular Epidemiology Molecular epidemiology biology Poverty-related infectious diseases [N4i 3] Mycobacteriology and Aerobic Actinomycetes bacterial infections and mycoses biology.organism_classification Molecular Typing Variable number tandem repeat Mycobacterium tuberculosis complex DNA Transposable Elements Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of clinical microbiology, 51(4), 1193-1198. American Society for Microbiology Journal of Clinical Microbiology, 51, 1193-8 Journal of Clinical Microbiology, 51, 4, pp. 1193-8 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | In order to switch from IS 6110 and polymorphic GC-rich repetitive sequence (PGRS) restriction fragment length polymorphism (RFLP) to 24-locus variable-number tandem-repeat (VNTR) typing of Mycobacterium tuberculosis complex isolates in the national tuberculosis control program in The Netherlands, a detailed evaluation on discriminatory power and agreement with findings in a cluster investigation was performed on 3,975 tuberculosis cases during the period of 2004 to 2008. The level of discrimination of the two typing methods did not differ substantially: RFLP typing yielded 2,733 distinct patterns compared to 2,607 in VNTR typing. The global concordance, defined as isolates labeled unique or identically distributed in clusters by both methods, amounted to 78.5% ( n = 3,123). Of the remaining 855 cases, 12% ( n = 479) of the cases were clustered only by VNTR, 7.7% ( n = 305) only by RFLP typing, and 1.8% ( n = 71) revealed different cluster compositions in the two approaches. A cluster investigation was performed for 87% ( n = 1,462) of the cases clustered by RFLP. For the 740 cases with confirmed or presumed epidemiological links, 92% were concordant with VNTR typing. In contrast, only 64% of the 722 cases without an epidemiological link but clustered by RFLP typing were also clustered by VNTR typing. We conclude that VNTR typing has a discriminatory power equal to IS 6110 RFLP typing but is in better agreement with findings in a cluster investigation performed on an RFLP-clustering-based cluster investigation. Both aspects make VNTR typing a suitable method for tuberculosis surveillance systems. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|