Lamina propria mononuclear cells express and respond to interleukin-2 differently in Crohn's disease and ulcerative colitis
| Autor: | Jun Ichi Haruta, Takehiko Hosokawa, Masataka Shinoda, Takafumi Ando, Mitsune Tanimoto, Kazuo Kusugami, Kenji Ina |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Interleukin 2
Adult Male Adolescent CD3 Complex CD3 chemical and pharmacologic phenomena Inflammatory bowel disease Peripheral blood mononuclear cell Crohn Disease Internal Medicine medicine Humans RNA Messenger Colitis Intestinal Mucosa Killer Cells Lymphokine-Activated Lamina propria Lymphokine-activated killer cell biology business.industry General Medicine Middle Aged medicine.disease Ulcerative colitis CD56 Antigen medicine.anatomical_structure Immunology biology.protein Leukocytes Mononuclear Interleukin-2 Colitis Ulcerative Female business medicine.drug |
| Zdroj: | Internal medicine (Tokyo, Japan). 35(9) |
| ISSN: | 0918-2918 |
| Popis: | Lymphokine-activated killer (LAK) cell activity and messenger RNA (mRNA) expression for interleukin-2 (IL-2) were analyzed using lamina propria mononuclear cells (LPMC) in inflammatory bowel disease patients. Compared with control LPMC, Crohn's disease (CD) LPMC exhibited significantly higher levels of LAK cell activity, whereas ulcerative colitis (UC) cells showed significantly lower levels of cytolytic activity with a difference in the frequency of CD3+, CD56+ and CD3+, CD56+ LAK precursor cells. After incubation with IL-2, the proportion of CD3+, CD56+ lymphocytes continued to be higher in CD cultures and substantially lower in UC cultures. Freshly isolated CD LPMC exhibited significantly higher levels of IL-2 mRNA than controls. However, no significant difference was observed between UC and control cells. The level of IL-2 expression or responsiveness to IL-2 may be responsible for different mucosal immune reactivity between CD and UC patients. |
| Databáze: | OpenAIRE |
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