On the spin and valence state of iron in native soybean lipoxygenase-1
| Autor: | Johannes F.G. Vliegenthart, Steven Slappendel, Anders Ehrenberg, Bo G. Malmström, Leif Petersson, Gerrit A. Veldink |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Magnetic Resonance Spectroscopy
Chemical Phenomena Proton Iron Lipoxygenase Biophysics Soybean lipoxygenase 1 Biochemistry Bohr magneton Magnetics Paramagnetism symbols.namesake Nuclear magnetic resonance Molecular Biology Valence (chemistry) biology Chemistry Cell Biology Nuclear magnetic resonance spectroscopy Magnetic susceptibility Crystallography biology.protein symbols Soybeans |
| Zdroj: | Biochemical and Biophysical Research Communications. 108:673-677 |
| ISSN: | 0006-291X |
| Popis: | Measurements of the magnetic susceptibility of native lipoxygenase-1 yielded a value for the effective Bohr magneton number, n eff , equal to 5.2 which is characteristic for iron in high-spin Fe(II) state. Upon addition of native lipoxygenase-1 to a butanol-1/D 2 O solution a differential line-broadening of the proton resonances in the NMR spectrum of butanol-1 was observed due to relaxation enhancement from interaction between the paramagnetic iron and the protons. This finding excludes the possibility that the iron in native lipoxygenase-1 is in the low-spin Fe(II) state. These results are consistent with the proposed mechanism of the catalytic function of the iron in lipoxygenase (De Groot, J.J.M.C., Veldink, G.A., Vliegenthart, J.F.G., Boldingh, J., Wever, R. and Van Gelder, B.F. (1975) Biochim. Biophys. Acta 377, 71–79). |
| Databáze: | OpenAIRE |
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