Newcastle disease virus infection induces activation of the NLRP3 inflammasome
| Autor: | Dandan Li, Chan Ding, Lei Tan, Liao Ying, Cuiping Song, Yang Wang, Chunchun Meng, Yuan Zhan, Binbin Wang, Mao Xiang, Jie Zhu, Xusheng Qiu, Guangxun Meng, Yingjie Sun |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Inflammasomes Newcastle Disease animal diseases viruses Interleukin-1beta Newcastle disease virus Caspase 1 Biology Virus Replication Virus Cell Line Mice 03 medical and health sciences AIM2 Immune system Virology NLR Family Pyrin Domain-Containing 3 Protein medicine Animals Humans Mice Knockout Innate immune system Macrophages Inflammasome Macrophage Activation biochemical phenomena metabolism and nutrition Caspase Inhibitors Oncolytic virus Disease Models Animal 030104 developmental biology Viral replication Female medicine.drug |
| Zdroj: | Virology. 496:90-96 |
| ISSN: | 0042-6822 |
| Popis: | Inflammatory responses are important aspects of the innate immune system during virus infection. We found that Newcastle disease virus can induce inflammasome activation in the human macrophage-like cell line THP-1. Viral replication was required for inflammasome activation, and small hairpin RNA knockdown experiments indicated that IL-1β secretion was mediated by the NLRP3 inflammasome. We also verified the results in LPS-primed bone marrow-derived macrophages (BMDMs) from NLRP3-deficient and wild type mice. NDV is considered to be a promising oncolytic virus. Stimulating the immune system has been proposed as a key mechanism of oncolytic specificity, and the inflammasome appears to be an important mechanism by which NDV is controlled. Knockdown of inflammasome components or chemical inhibition of caspase-1 activity shows that cell survival was augmented and benefited NDV replication. This study shows that NLRP3 inflammasome activation is an innate cellular response to NDV infection and offers insights into the oncolytic specificity of NDV. |
| Databáze: | OpenAIRE |
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