Metabolic control through ornithine and uracil of epithelial cell invasion by Shigella flexneri
| Autor: | Jérôme M. B. Durand, Glenn R. Björk |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Ornithine
Virulence Factors Homoserine Virulence Environment Biology Hemolysis Microbiology Shigella flexneri Type three secretion system chemistry.chemical_compound Humans Uracil Pathogen Dysentery Bacillary Methionine Effector Epithelial Cells Gene Expression Regulation Bacterial biology.organism_classification chemistry Biochemistry Host-Pathogen Interactions HeLa Cells |
| Zdroj: | Microbiology. 155:2498-2508 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/mic.0.028191-0 |
| Popis: | This paper shows that compounds in defined growth media strongly influence the expression of the effectors of virulence in the human invasive pathogenShigella flexneri. Ornithine in conjunction with uracil reduces the haemolytic ability of wild-type cultures more than 20-fold and the expression of the type III secretion system more than 8-fold, as monitored by anmxiC : : lacZtranscriptional reporter.mxiCgene expression is further decreased by the presence of methionine or branched-chain amino acids (15-fold or 25-fold at least, respectively). Lysine and a few other aminated metabolites (cadaverine, homoserine and diaminopimelate) counteract the ornithine-mediated inhibition of haemolytic activity and of the expression of a transcriptional activatorvirFreporter. The complete abolition of invasion of HeLa cells by wild-type bacteria by ornithine, uracil, methionine or branched-chain amino acids establishes that these metabolites are powerful effectors of virulence. These findings provide a direct connection between metabolism and virulence inS. flexneri. The inhibitory potential exhibited by the nutritional environment is stronger than temperature, the classical environmental effector of virulence. The implications and practical application of this finding in prophylaxis and treatment of shigellosis are discussed. |
| Databáze: | OpenAIRE |
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