Membrane fatty acid composition as a determinant of Listeria monocytogenes sensitivity to trans-cinnamaldehyde
| Autor: | Biniam Tamiru Kebede, Chris W. Michiels, Gil Rogiers, Ann Van Loey |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Membrane Fluidity Transamination 030106 microbiology Mutant Biology medicine.disease_cause Microbiology Cinnamaldehyde 03 medical and health sciences chemistry.chemical_compound Anti-Infective Agents Bacterial Proteins Listeria monocytogenes Membrane fluidity medicine Acrolein Molecular Biology chemistry.chemical_classification Cell Membrane Fatty Acids Genetic Complementation Test Fatty acid General Medicine Culture Media Amino acid Complementation 030104 developmental biology chemistry Biochemistry Mutation Food Preservatives Amino Acids Branched-Chain |
| Popis: | Trans-cinnamaldehyde, the major compound of cinnamon essential oil, is a potentially interesting natural antimicrobial food preservative. Although a number of studies have addressed its mode of action, the factors that determine bacterial sensitivity or tolerance to trans-cinnamaldehyde are poorly understood. We report the detailed characterization of a Listeria monocytogenes Scott A trans-cinnamaldehyde hypersensitive mutant defective in IlvE, which catalyzes the reversible transamination of branched-chain amino acids to the corresponding short-chain α-ketoacids. This mutant showed an 8.4 fold extended lag phase during growth in sublethal concentrations (4 mM), and faster inactivation in lethal concentrations of trans-cinnamaldehyde (6 mM). Trans-cinnamaldehyde hypersensitivity could be corrected by genetic complementation with the ilvE gene and supplementation with branched-chain α-ketoacids. Whole-cell fatty acid analyses revealed an almost complete loss of anteiso branched-chain fatty acids (BCFAs), which was compensated by elevated levels of unbranched saturated fatty acids and iso-BCFAs. Sub-inhibitory concentrations of trans-cinnamaldehyde induced membrane fatty acid adaptations predicted to reduce membrane fluidity, possibly as a response to counteract the membrane fluidizing effect of trans-cinnamaldehyde. These results demonstrate the role of IlvE in BCFA production and the role of membrane composition as an important determinant of trans-cinnamaldehyde sensitivity in L. monocytogenes. ispartof: Research in Microbiology vol:168 issue:6 pages:536-546 ispartof: location:France status: published |
| Databáze: | OpenAIRE |
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