Characterisation of the Antibiotic Profile of Lysobacter capsici AZ78, an Effective Biological Control Agent of Plant Pathogenic Microorganisms
| Autor: | Gerardo Puopolo, Bernard Seidl, Anthi Vlassi, Francesca Brescia, Ana Bejarano, Rainer Schuhmacher, Martina Marchetti-Deschmann |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) biopesticides Biopesticides Lysobacter capsici WAP-8294A2 QH301-705.5 Microorganism biological control UHPLC-HRMS/MS 5-diketopiperazines Lysobacter dihydromaltophilin (HSAF) 01 natural sciences Microbiology Article 2 5-diketopiperazines 03 medical and health sciences Rhodococcus fascians Virology Metabolome Biology (General) 2. Zero hunger biology 010405 organic chemistry biology.organism_classification Antimicrobial 0104 chemical sciences Pythium ultimum 030104 developmental biology MALDI-qTOF-MSI Biological control Plasmopara viticola Settore AGR/12 - PATOLOGIA VEGETALE Bacteria |
| Zdroj: | Microorganisms, Vol 9, Iss 1320, p 1320 (2021) Microorganisms Volume 9 Issue 6 |
| Popis: | Determining the mode of action of microbial biocontrol agents plays a key role in their development and registration as commercial biopesticides. The biocontrol rhizobacteriumLysobacter capsiciAZ78 (AZ78) is able to inhibit a vast array of plant pathogenic oomycetes and Gram-positive bacteria due to the release of antimicrobial secondary metabolites. A combination of MALDI-qTOF-MSI and UHPLC-HRMS/M was applied to finely dissect the AZ78 metabolome and identify the main secondary metabolites involved in the inhibition of plant pathogenic microorganisms. Under nutritionally limited conditions, MALDI-qTOF-MSI revealed that AZ78 is able to release a relevant number of antimicrobial secondary metabolites belonging to the families of 2,5-diketopiperazines, cyclic lipodepsipeptides, macrolactones and macrolides. In vitro tests confirmed the presence of secondary metabolites toxic againstPythium ultimumandRhodococcus fasciansin AZ78 cell-free extracts. Subsequently, UHPLC-HRMS/MS was used to confirm the results achieved with MALDI-qTOF-MSI and investigate for further putative antimicrobial secondary metabolites known to be produced byLysobacterspp. This technique confirmed the presence of several 2,5-diketopiperazines in AZ78 cell-free extracts and provided the first evidence of the production of the cyclic depsipeptide WAP-8294A2 in a member ofL. capsicispecies. Moreover, UHPLC-HRMS/MS confirmed the presence of dihydromaltophilin/Heat Stable Antifungal Factor (HSAF) in AZ78 cell-free extracts. Due to the production of HSAF by AZ78, cell-free supernatants were effective in controllingPlasmopara viticolaon grapevine leaf disks after exposure to high temperatures. Overall, our work determined the main secondary metabolites involved in the biocontrol activity of AZ78 against plant pathogenic oomycetes and Gram-positive bacteria. These results might be useful for the future development of this bacterial strain as the active ingredient of a microbial biopesticide that might contribute to a reduction in the chemical input in agriculture. |
| Databáze: | OpenAIRE |
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