Fat feeding impairs glycogen synthase activity in mice without effects on its gene expression
| Autor: | Esa Laurila, Bo Ahrén, Xudong Huang, Leif Groop, Mona Hansson |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Blood Glucose
medicine.medical_specialty Ratón Endocrinology Diabetes and Metabolism medicine.medical_treatment Gene Expression Regulation Enzymologic Pathogenesis Mice Endocrinology Internal medicine Diabetes mellitus Gene expression Mole Diabetes Mellitus medicine Animals Insulin RNA Messenger Muscle Skeletal Glycogen synthase chemistry.chemical_classification biology Body Weight medicine.disease Dietary Fats Diet Mice Inbred C57BL Glycogen Synthase Enzyme chemistry biology.protein Female |
| Zdroj: | Metabolism. 52:535-539 |
| ISSN: | 0026-0495 |
| DOI: | 10.1053/meta.2003.50107 |
| Popis: | To examine whether the effects of high-fat feeding on glycogen synthase (GS) activity and mRNA levels differ between diabetes-prone (C57BL/6J) and diabetes-resistant mice (NMRI), we measured GS activity and mRNA levels in muscle from C57BL/6J and NMRI mice fed a high-fat or normal chow diet for 3, 6, and 15 months. As compared with chow feeding, fat feeding increased plasma insulin levels in C57BL/6J mice at 15 months (464 +/- 29 v 267 +/- 47 pmol/L, P =.005), which was associated with elevated plasma glucose levels at 15 months (5.3 +/- 0.3 v 3.8 +/- 0.2 mmol/L, P =.001). Fat feeding increased plasma insulin levels also in NMRI mice at 15 months (705 +/- 145 v 275 +/- 64 pmol/L, P =.01) without, however, a rise of plasma glucose levels. In parallel with increased insulin levels, decreased muscle GS fractional velocity (FV) was observed at 6 (49.0% +/- 2.6% v 69.1% +/- 7.3%, P =.04) and 15 (45.8% +/- 1.8% v 53.4% +/- 1.6 %, P |
| Databáze: | OpenAIRE |
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