Circular RNA TGFBR2 acts as a ceRNA to suppress nasopharyngeal carcinoma progression by sponging miR-107

Autor: Weidong Zhao, Houyong Li, Xianhui Ning, Quan Liu, Hanyu Lu, Jingjing Wang, Xicai Sun, Dehui Wang, Yurong Gu, Huan Wang, Li Hu, Huankang Zhang, Zhuofu Liu, Wanpeng Li
Rok vydání: 2021
Předmět:
Male
0301 basic medicine
Cancer Research
Microarray
Datasets as Topic
Down-Regulation
Respiratory Mucosa
Biology
Mice
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Cell Movement
Circular RNA
Cell Line
Tumor
Nasopharynx
microRNA
Biomarkers
Tumor
otorhinolaryngologic diseases
medicine
Animals
Humans
RNA
Small Interfering
Cell Proliferation
Messenger RNA
Nasopharyngeal Carcinoma
Competing endogenous RNA
Gene Expression Profiling
Receptor
Transforming Growth Factor-beta Type II
Nasopharyngeal Neoplasms
RNA
Circular
Prognosis
medicine.disease
Xenograft Model Antitumor Assays
In vitro
Up-Regulation
Gene Expression Regulation
Neoplastic
MicroRNAs
stomatognathic diseases
030104 developmental biology
Oncology
Nasopharyngeal carcinoma
Gene Knockdown Techniques
030220 oncology & carcinogenesis
Cancer research
Signal Transduction
Zdroj: Cancer Letters. 499:301-313
ISSN: 0304-3835
DOI: 10.1016/j.canlet.2020.11.001
Popis: Circular RNAs (circRNAs) act as competing endogenous RNAs, which are involved in the regulation of many types of cancers. They primarily function by sponging microRNAs (miRNAs) and influencing the expression of miRNA by target messenger RNA. However, the role of circRNAs in the progression of nasopharyngeal carcinoma (NPC) remains largely unclear. In this study, differentially expressed miRNAs associated with NPC were screened using microarray analyses, from which miR-107 was identified. Increased miR-107 expression was associated with poor prognosis in NPC, and miR-107 promoted the proliferation and migration of NPC cells. TGFBR2 was identified as the direct target of miR-107, which could reverse its effect on NPC cells. Furthermore, the expression of circTGFBR2 was downregulated in NPC tissue samples, while circTGFBR2 overexpression correlated with favorable prognosis in NPC. Functionally, circTGFBR2 overexpression inhibited the proliferation and migration of NPC cells both in vitro and in vivo. Further analysis showed that circTGFBR2 sponged miR-107, leading to the upregulation of TGFBR2 expression and suppression of NPC progression. Therefore, circTGFBR2 may serve as a novel tumor suppressive factor and potential target for new therapies in NPC patients.
Databáze: OpenAIRE
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