Size-dependent effects of gold nanoparticles uptake on maturation and antitumor functions of human dendritic cells in vitro
| Autor: | Srđa Janković, Dragana Vučević, Bernd Friedrich, Jelena Rajković, Sergej Tomić, Miodrag Čolić, Nina Ogrinc, Marjan Slak Rupnik, Sasa Vasilijic, Petar Milosavljevic, Rebeka Rudolf, Primož Pelicon, Ivan Anžel, Jelena Đokić |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Lipopolysaccharides
T-Lymphocytes lcsh:Medicine 02 engineering and technology law.invention Analytical Chemistry Spectrum Analysis Techniques Cell Signaling law Animal Cells Molecular Cell Biology Medicine Nanotechnology lcsh:Science Cells Cultured 0303 health sciences Multidisciplinary medicine.diagnostic_test Size dependent Cell Polarity 021001 nanoscience & nanotechnology Flow Cytometry Interleukin-12 3. Good health Cell biology Chemistry Colloidal gold Research Design Spectrophotometry Physical Sciences Engineering and Technology Cytophotometry Cellular Types 0210 nano-technology Research Article Signal Transduction Tumor Immunology Immune Cells Immunology Materials Science Antigen-Presenting Cells Antineoplastic Agents Research and Analysis Methods Flow cytometry 03 medical and health sciences Th2 Cells Chemical Analysis Confocal microscopy Humans Calcium Signaling Particle Size Materials by Attribute 030304 developmental biology Nanomaterials business.industry lcsh:R Quantitative Analysis Biology and Life Sciences Cell Biology Dendritic Cells In vitro Apoptosis Nanoparticles Th17 Cells Necrotic tumor lcsh:Q Gold Electron microscope business Cytometry T-Lymphocytes Cytotoxic |
| Zdroj: | PLoS ONE, Vol 9, Iss 5, p e96584 (2014) PLOS ONE 9(5), e96584 (2014). doi:10.1371/journal.pone.0096584 PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Gold nanoparticles (GNPs) are claimed as outstanding biomedical tools for cancer diagnostics and photo-thermal therapy, but without enough evidence on their potentially adverse immunological effects. Using a model of human dendritic cells (DCs), we showed that 10 nm- and 50 nm-sized GNPs (GNP10 and GNP50, respectively) were internalized predominantly via dynamin-dependent mechanisms, and they both impaired LPS-induced maturation and allostimulatory capacity of DCs, although the effect of GNP10 was more prominent. However, GNP10 inhibited LPS-induced production of IL-12p70 by DCs, and potentiated their Th2 polarization capacity, while GNP50 promoted Th17 polarization. Such effects of GNP10 correlated with a stronger inhibition of LPS-induced changes in Ca2+ oscillations, their higher number per DC, and more frequent extra-endosomal localization, as judged by live-cell imaging, proton, and electron microscopy, respectively. Even when released from heat-killed necrotic HEp-2 cells, GNP10 inhibited the necrotic tumor cell-induced maturation and functions of DCs, potentiated their Th2/Th17 polarization capacity, and thus, impaired the DCs' capacity to induce T cell-mediated anti-tumor cytotoxicity in vitro. Therefore, GNP10 could potentially induce more adverse DC-mediated immunological effects, compared to GNP50. |
| Databáze: | OpenAIRE |
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