Folic Acid Functionalized Surface Highlights 5-Methylcytosine-Genomic Content within Circulating Tumor Cells
| Autor: | Patrizio Candeloro, Natalia Malara, Enzo Di Fabrizio, Tania Limongi, M. Renne, Maria Laura Coluccio, Ubaldo Prati, Gheorghe Cojoc, Stefania De Vitis, Cinzia Raso, Monica Asande, Raffaella Raimondo, Gerardo Perozziello, Vincenzo Mollace, Laura Roveda, Valentina Trunzo |
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| Rok vydání: | 2014 |
| Předmět: |
Surface Properties
Cells Cell Enzyme-Linked Immunosorbent Assay Biology Neoplastic Cells Biomaterials functionalized surfaces chemistry.chemical_compound Folic Acid Circulating tumor cell folic acid folic acid receptors hyper-methylated circulating tumor cells 5-Methylcytosine Biomarkers Tumor Blood Chemical Analysis Cells Cultured DNA Methylation Genes Neoplasm Humans Microscopy Confocal Neoplasms Neoplastic Cells Circulating Survival Analysis Circulating medicine General Materials Science Survival analysis Microscopy Tumor Cultured Cancer General Chemistry Methylation medicine.disease medicine.anatomical_structure Genes chemistry Cell-free fetal DNA Confocal DNA methylation Immunology Cancer research Neoplasm Biomarkers Biotechnology |
| Zdroj: | Small. |
| ISSN: | 1613-6810 |
| Popis: | Although the detection of methylated cell free DNA represents one of the most promising approaches for relapse risk assessment in cancer patients, the low concentration of cell-free circulating DNA constitutes the biggest obstacle in the development of DNA methylation-based biomarkers from blood. This paper describes a method for the measurement of genomic methylation content directly on circulating tumor cells (CTC), which could be used to deceive the aforementioned problem. Since CTC are disease related blood-based biomarkers, they result essential to monitor tumor's stadiation, therapy, and early relapsing lesions. Within surface's bio-functionalization and cell's isolation procedure standardization, the presented approach reveals a singular ability to detect high 5-methylcytosine CTC-subset content in the whole CTC compound, by choosing folic acid (FA) as transducer molecule. Sensitivity and specificity, calculated for FA functionalized surface (FA-surface), result respectively on about 83% and 60%. FA-surface, allowing the detection and characterization of early metastatic dissemination, provides a unique advance in the comprehension of tumors progression and dissemination confirming the presence of CTC and its association with high risk of relapse. This functionalized surface identifying and quantifying high 5-methylcytosine CTC-subset content into the patient's blood lead significant progress in cancer risk assessment, also providing a novel therapeutic strategy. |
| Databáze: | OpenAIRE |
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