Transformation of MCF-10A Human Breast Epithelial Cells by Zeranol and Estradiol-17beta
| Autor: | Young C. Lin, Suling Liu |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Breast Neoplasms medicine.disease_cause chemistry.chemical_compound Breast cancer Internal medicine Internal Medicine medicine Zeranol Humans Potency Doubling time Neoplastic transformation Breast Estrogens Non-Steroidal skin and connective tissue diseases Estrogen receptor beta Cell Proliferation DNA Primers Dose-Response Relationship Drug Estradiol Reverse Transcriptase Polymerase Chain Reaction business.industry Epithelial Cells medicine.disease Cell Transformation Neoplastic Endocrinology Oncology chemistry Estrogen Cancer research RNA Female Surgery Carcinogenesis business |
| Zdroj: | The Breast Journal. 10:514-521 |
| ISSN: | 1524-4741 1075-122X |
| DOI: | 10.1111/j.1075-122x.2004.21410.x |
| Popis: | Among the endocrine factors associated with breast cancer, estrogens are considered to play a central role in human breast carcinogenesis. Breast cancer risks are increased by long-term exposure to estrogens. Zeranol (Ralgro) is a nonsteroidal agent with estrogenic activity that is used as a growth promoter in the U.S. beef and veal industry. To determine whether zeranol and estradiol-17beta play a role in the neoplastic transformation of human breast and to compare the estrogenic potency of zeranol to that of estradiol-17beta in human breast, we treated human breast epithelial cell MCF-10A with different doses of zeranol or estradiol-17beta for 10 repeated treatment cycles. By utilizing the doubling time assay, soft agar assay, and reverse transcriptase polymerase chain reaction (RT-PCR) assay, we showed that 10 repeated estradiol-17beta or zeranol treatment cycles to MCF-10A cells decrease the doubling time of the cells by 30 to 40% and stimulate colony formation in soft agar and induce estrogen receptor beta (ER-beta) mRNA expression, all of which are not dose related in our tested dose range. Furthermore, we show that zeranol and estradiol-17beta have a similar potency in the stimulation and inhibition of gene expressions in human breast cancer cell line MCF-7 by RT-PCR. These results indicate that both zeranol and estradiol-17beta can induce human breast epithelial cell neoplastic transformation with similar potency in the long-term exposure through the oxidation-reduction (redox) pathway and/or ER-beta-mediated pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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