Pharmacokinetics of 13 active components in a rat model of middle cerebral artery occlusion after intravenous injection of Radix Salviae miltiorrhizae-Lignum dalbergiae odoriferae prescription
| Autor: | Ye Zhao, Sha Liao, Yi‐fei Li, Shixiang Wang, Rui Zhao, Ying Feng, Qiannan Li, Baimei Shi, Jie Yu, Xufen Dai, Xiaohui Zheng, Pu Jia |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Drug Compounding Biological Availability Filtration and Separation Salvia miltiorrhiza Pharmacology 01 natural sciences High-performance liquid chromatography Analytical Chemistry Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Pharmacokinetics Tandem Mass Spectrometry Caffeic acid Animals Radix Chromatography High Pressure Liquid 030304 developmental biology 0303 health sciences Rosmarinic acid 010401 analytical chemistry Area under the curve Infarction Middle Cerebral Artery 0104 chemical sciences Bioavailability Rats Disease Models Animal Drug Combinations chemistry Injections Intravenous Protocatechuic aldehyde Drugs Chinese Herbal |
| Zdroj: | Journal of separation scienceREFERENCES. 43(2) |
| ISSN: | 1615-9314 |
| Popis: | As a representative formulation of Radix Salviae miltiorrhizae (Danshen)-Lignum Dalbergiae odoriferae (Jiangxiang), Xiangdan injection is widely prescribed for cardio- and cerebrovascular diseases in practice. This necessitates a pharmacokinetic investigation of this formulation to make it safer and more broadly applicable. We developed and validated a sensitive, selective, and reliable high-performance liquid chromatography with tandem mass spectrometry method for the simultaneous determination of 11 phenolic compounds including danshensu plus two diterpenoid quinones like cryptotanshinone and tanshinone IIA in rat. We applied this method for the pharmacokinetic studies of the 13 compounds in a rat model of middle cerebral artery occlusion after intravenous injection of Xiangdan injection or Danshen injection. In sham-operated rats, the animals taking Xiangdan injection exhibited significant growth of the area under the curve for danshensu, protocatechuic aldehyde, and tanshinone IIA compared with the changes seen in the data of those administrated with Danshen injection. Such a pattern was also observed in middle cerebral artery occlusion rats, whereas increased the area under the curve values were observed for danshensu, protocatechuic aldehyde, caffeic acid, rosmarinic acid, and tanshinone IIA. These results demonstrated that synergistic interactions occurred between the components of Danshen and the active compounds of Jiangxiang both in sham-operated and middle cerebral artery occlusion rats, increasing the bioavailability of Danshen. The results presented herein can be used to determine a reference dose for the clinical application of Xiangdan injection, and to elucidate the synergistic mechanism of Danshen and Jiangxiang. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text