OsATL38 mediates mono-ubiquitination of the 14-3-3 protein OsGF14d and negatively regulates the cold stress response in rice
| Autor: | Mi Young Byun, Tae Rin Oh, Andosung Lee, Hee Woong Yang, Woo Taek Kim, Hye Jo Min, Seong Guan Yu, Li Hua Cui |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Physiology
Ubiquitin-Protein Ligases Transgene Mutant Plant Science Ubiquitin Gene Expression Regulation Plant Stress Physiological Arabidopsis 14-3-3 protein Plant Proteins chemistry.chemical_classification Gene knockdown DNA ligase biology Chemistry Cold-Shock Response Ubiquitination food and beverages Oryza Plants Genetically Modified biology.organism_classification Genetically modified rice Cell biology 14-3-3 Proteins biology.protein |
| Zdroj: | Journal of Experimental Botany. 73:307-323 |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | One of the major regulatory pathways that permits plants to convert an external stimulus into an internal cellular response within a short period of time is the ubiquitination pathway. In this study, OsATL38 was identified as a low temperature-induced gene that encodes a rice homolog of Arabidopsis Tóxicos en Levadura RING-type E3 ubiquitin (Ub) ligase, which was predominantly localized to the plasma membrane. OsATL38-overexpressing transgenic rice plants exhibited decreased tolerance to cold stress as compared with wild-type rice plants. In contrast, RNAi-mediated OsATL38 knockdown transgenic progeny exhibited markedly increased tolerance to cold stress relative to that of wild-type plants, which indicated a negative role of OsATL38 in response to cold stress. Yeast two-hybrid, in vitro pull-down, and co-immunoprecipitation assays revealed that OsATL38 physically interacted with OsGF14d, a rice 14-3-3 protein. An in vivo target ubiquitination assay indicated that OsGF14d was mono-ubiquitinated by OsATL38. osgf14d knockout mutant plants were more sensitive to cold stress than wild-type rice plants, indicating that OsGF14d is a positive factor in the response to cold stress. These results provide evidence that the RING E3 Ub ligase OsATL38 negatively regulates the cold stress response in rice via mono-ubiquitination of OsGF14d 14-3-3 protein. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|