Colloidal Stability and Surface Chemistry Are Key Factors for the Composition of the Protein Corona of Inorganic Gold Nanoparticles
| Autor: | Wolfgang G. Kreyling, Simon Ristig, Stefan Thalhammer, Martin Schäffler, Christian Pfeiffer, Matthias Epple, Jonas Hühn, Stefanie M. Hauck, Stephanie Hirn, Wolfgang J. Parak, Hakan Sarioglu, Pablo del Pino, Blair D Johnston, Nadine Haberl, Manuela Semmler-Behnke |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
endocrine system
Chemie Nanoparticle Protein Corona 02 engineering and technology Polyethylene glycol Conjugated system 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 0104 chemical sciences Electronic Optical and Magnetic Materials Biomaterials chemistry.chemical_compound Colloid chemistry Chemical engineering Colloidal gold Electrochemistry Surface modification Organic chemistry 0210 nano-technology Citric acid |
| Popis: | To study the influence of colloidal stability on protein corona formation, gold nanoparticles are synthesized with five distinct surface modifications: coating with citric acid, bis(p-sulfonatophenyl)phenylphosphine dihydrate dipotassium salt, thiol-terminated methoxy-polyethylene glycol, dodecylamine-grafted poly(isobutylene-alt-maleic anhydride), and dodecylamine-grafted poly(isobutylene-alt-maleic anhydride) conjugated with polyethylene glycol. The nanoparticles are incubated with serum or bronchoalveolar lavage fluid from C57BL/6 mice (15 min or 24 h) to assess the effect of differential nanoparticle surface presentation on protein corona formation in the air–blood barrier exposure pathway. Proteomic quantification and nanoparticle size measurements are used to assess protein corona formation. We show that surface modification has a clear effect on the size and the composition of the protein corona that is related to the colloidal stability of the studied nanoparticles. Additionally, differences in the composition and size of the protein corona are shown between biological media and duration of exposure, indicating evolution of the corona through this exposure pathway. Consequently, a major determinant of protein corona formation is the colloidal stability of nanoparticles in biological media and chemical or environmental modification of the nanoparticles alters the surface presentation of the functional epitope in vivo. Therefore, the colloidal stability of nanoparticles has a decisive influence on nano–bio interactions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|