Nuclear magnetic resonance investigation of water transport through the plasma membrane of various yeast species
| Autor: | Hana Elicharová, Pavel Srb, Mária Šoltésová, Michal Růžička, Larisa Janisova, Jan Lang, Hana Sychrová |
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| Rok vydání: | 2019 |
| Předmět: |
Magnetic Resonance Spectroscopy
Membrane permeability Osmotic shock Saccharomyces cerevisiae Aquaporins Osmosis Microbiology 03 medical and health sciences Nuclear magnetic resonance Candida albicans Schizosaccharomyces Genetics Molecular Biology 030304 developmental biology 0303 health sciences Water transport biology 030306 microbiology Chemistry Cell Membrane Temperature Zygosaccharomyces Water Biological Transport Nuclear magnetic resonance spectroscopy biology.organism_classification Yeast Kinetics Membrane Thermodynamics |
| Zdroj: | FEMS Microbiology Letters. 366 |
| ISSN: | 1574-6968 0378-1097 |
| Popis: | A specific technique of nuclear magnetic resonance (NMR) spectroscopy, filter-exchange spectroscopy (FEXSY), was employed to investigate water transport through the plasma membrane in intact yeast cells. This technique allows water transport to be monitored directly, thus avoiding the necessity to subject the cells to any rapid change in the external conditions, e.g. osmotic shock. We established a sample preparation protocol, a data analysis procedure and verified the applicability of FEXSY experiments. We recorded the exchange rates in the temperature range 10–40°C for Saccharomyces cerevisiae. The resulting activation energy of 29 kJ mol−1 supports the hypothesis that water exchange is facilitated by water channels—aquaporins. Furthermore, we measured for the first time water exchange rates in three other phylogenetically unrelated yeast species (Schizosaccharomyces pombe, Candida albicans and Zygosaccharomyces rouxii) and observed remarkably different water exchange rates between these species. Findings of our work contribute to a better understanding of as fundamental a cell process as the control of water transport through the plasma membrane. |
| Databáze: | OpenAIRE |
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