5-HT2a receptors in rat sciatic nerves and Schwann cell cultures
| Autor: | Chun Wu, Areum Han, T. J. Deerinck, Karen Kinder, Elizabeth J. Yoder, Abraham Hanono, Mark H. Ellisman, Guido M. Gaietta |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Serotonin
Histology Schwann cell Endogeny Biology Nerve Fibers Myelinated Rats Sprague-Dawley medicine Animals Receptor Serotonin 5-HT2A Mast Cells Peripheral Nerves RNA Messenger Receptor Cells Cultured Myelin Sheath Messenger RNA General Neuroscience Macrophages Cell Membrane Cell Biology Mast cell Immunohistochemistry Sciatic Nerve Cell biology Rats Chemotaxis Leukocyte Microscopy Electron medicine.anatomical_structure nervous system Animals Newborn Cytoplasm biology.protein Sciatic nerve Schwann Cells Anatomy Antibody Neuroscience Demyelinating Diseases |
| Zdroj: | Journal of neurocytology. 32(4) |
| ISSN: | 0300-4864 |
| Popis: | Pharmacological approaches and optical recordings have shown that Schwann cells of a myelinating phenotype are activated by 5-HT upon its interaction with the 5-HT(2A) receptor (5-HT(2A)R). In order to further characterize the expression and distribution of this receptor in Schwann cells, we examined rat sciatic nerve and cultured rat Schwann cells using probes specific to 5-HT(2A)R protein mRNA. We also examined the endogenous sources of 5-HT in rat sciatic nerve by employing both histochemical stains and an antibody that specifically recognizes 5-HT. Rat Schwann cells of a myelinating phenotype contained both 5-HT(2A)R protein and mRNA. In the healthy adult rat sciatic nerve, 5-HT(2A)Rs were evenly distributed along the outermost portion of the Schwann cell plasma membrane and within the cytoplasm. The most prominent source of 5-HT was within granules of the endoneurial mast cells, closely juxtaposed to Schwann cells within myelinating sciatic nerves. These results support the hypothesis that the 5-HT receptors expressed by rat Schwann cells in vivo are activated by the release of 5-HT from neighboring mast cells. |
| Databáze: | OpenAIRE |
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