Using Amplicon Sequencing To Characterize and Monitor Bacterial Diversity in Drinking Water Distribution Systems
| Autor: | Paul Monis, Laura S. Weyrich, Alan Cooper, Emma Sawade, Mary Drikas, Jennifer L. A. Shaw |
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| Přispěvatelé: | Shaw, Jennifer LA, Monis, Paul, Weyrich, Laura S, Sawade, Emma, Drikas, Mary, Cooper, Alan J |
| Rok vydání: | 2015 |
| Předmět: |
gastrointestinal illnesses
Portable water purification Biology water quality Applied Microbiology and Biotechnology Water Purification Microbiology chemistry.chemical_compound Nitrate RNA Ribosomal 16S Water Quality Food science Bacteria Ecology Public and Environmental Health Microbiology Drinking Water Microbiota Chloramines drinking water Genes rRNA Sequence Analysis DNA biology.organism_classification Biota Nitrification Disinfection Burkholderiales chemistry drinking water distribution systems (DWDSs) Metagenomics Metagenome Microbial Interactions Water treatment Water quality Water Microbiology Nitrospira Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 81:6463-6473 |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | Drinking water assessments use a variety of microbial, physical, and chemical indicators to evaluate water treatment efficiency and product water quality. However, these indicators do not allow the complex biological communities, which can adversely impact the performance of drinking water distribution systems (DWDSs), to be characterized. Entire bacterial communities can be studied quickly and inexpensively using targeted metagenomic amplicon sequencing. Here, amplicon sequencing of the 16S rRNA gene region was performed alongside traditional water quality measures to assess the health, quality, and efficiency of two distinct, full-scale DWDSs: (i) a linear DWDS supplied with unfiltered water subjected to basic disinfection before distribution and (ii) a complex, branching DWDS treated by a four-stage water treatment plant (WTP) prior to disinfection and distribution. In both DWDSs bacterial communities differed significantly after disinfection, demonstrating the effectiveness of both treatment regimes. However, bacterial repopulation occurred further along in the DWDSs, and some end-user samples were more similar to the source water than to the postdisinfection water. Three sample locations appeared to be nitrified, displaying elevated nitrate levels and decreased ammonia levels, and nitrifying bacterial species, such as Nitrospira , were detected. Burkholderiales were abundant in samples containing large amounts of monochloramine, indicating resistance to disinfection. Genera known to contain pathogenic and fecal-associated species were also identified in several locations. From this study, we conclude that metagenomic amplicon sequencing is an informative method to support current compliance-based methods and can be used to reveal bacterial community interactions with the chemical and physical properties of DWDSs. |
| Databáze: | OpenAIRE |
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