Solventogenic enzymes of Clostridium acetobutylicum: catalytic properties, genetic organization, and transcriptional regulation
| Autor: | Peter Dürre, Uwe Sauer, Benjamin Stürzenhofecker, Anita Kuhn, Ralf-Jörg Fischer, Karin Lorenz, Klaus Winzer, Susanne Ullmann, Wiebke Schreiber |
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| Rok vydání: | 1995 |
| Předmět: |
Clostridium acetobutylicum
Transcription Genetic Operon Carboxy-Lyases Molecular Sequence Data Biology Microbiology Isozyme 03 medical and health sciences chemistry.chemical_compound Clostridium Acetoacetate decarboxylase 030304 developmental biology Alcohol dehydrogenase 0303 health sciences Base Sequence 030306 microbiology Thiolase Butanol Gene Expression Regulation Bacterial equipment and supplies biology.organism_classification Infectious Diseases chemistry Biochemistry biology.protein Solvents lipids (amino acids peptides and proteins) Coenzyme A-Transferases Oxidoreductases |
| Zdroj: | FEMS microbiology reviews. 17(3) |
| ISSN: | 0168-6445 |
| Popis: | The enzymes acetoacetate decarboxylase and coenzyme A transferase catalyse acetone production from acetoacetyl-CoA in Clostridium acetobutylicum. The adc gene encoding the former enzyme is organized in a monocistronic operon, while the ctf genes form a common transcription unit with the gene (adhE) encoding a probable polyfunctional aldehyde/alcohol dehydrogenase. This genetic arrangement could reflect physiological requirements at the onset of solventogenesis. In addition to AdhE, two butanol dehydrogenase isozymes and a thiolase are involved in butanol synthesis. RNA analyses showed a sequential order of induction for the different butanol dehydrogenase genes, indicating an in vivo function of BdhI in low level butanol formation. The physiological roles of AdhE and BdhII most likely involve high level butanol formation, with AdhE being responsible for the onset of solventogenesis and BdhII ensuring continued butanol production. Addition of methyl viologen results in artificially induced butanol synthesis which seems to be mediated by a still unknown set of enzymes. Although the signal that triggers the shift to solventogenesis has not yet been elucidated, recent investigations suggest a possible function of DNA supercoiling as a transcriptional sensor of the respective environmental stimuli. |
| Databáze: | OpenAIRE |
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