Evaluation of bioartificial renal tubule device prepared with lifespan-extended human renal proximal tubular epithelial cells
| Autor: | Satoshi Fujimura, Takashi Hirukawa, Kaichiro Sawada, Ryoko Tatsumi, Takatoshi Kakuta, Akira Saito, Hajime Suzuki, Masafumi Fukagawa, Takayo Miyakogawa, Noriyuki Sanechika, Hiroo Takahashi, Genta Kanai |
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| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Artificial kidney Kidney Function Tests Kidney Tubules Proximal chemistry.chemical_compound Internal medicine medicine Animals Humans Renal replacement therapy Blood urea nitrogen Cellular Senescence Transplantation Kidney Creatinine Bioartificial Organs business.industry Interleukin-6 Goats Acute kidney injury Epithelial Cells Acute Kidney Injury medicine.disease Nephrectomy Disease Models Animal medicine.anatomical_structure Endocrinology chemistry Nephrology Cytokines Multiple organ dysfunction syndrome business Biomarkers |
| Zdroj: | Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association. 27(8) |
| ISSN: | 1460-2385 |
| Popis: | BACKGROUND Acute kidney injury (AKI), accompanied by the development of systemic inflammatory response syndrome and multiorgan dysfunction syndrome, is associated with a high risk of death. Bioartificial renal tubule device (BTD) is a cell therapy that improves the conditions common to artificial kidney recipients treated for kidney diseases. In this paper, we describe the establishment of BTD with lifespan-extended human renal proximal tubular epithelial cells. METHODS AKI goats were established by performing bilateral nephrectomy followed by lipopolysaccharide administration. The AKI goats were treated with BTD or sham-BTD, and the two groups of animals were compared by measuring the respective life spans and the levels of blood urea nitrogen, creatinine, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and serum electrolytes. The expression levels of inflammatory cytokines were detected by reverse transcription-polymerase chain reaction, and plasma interleukin (IL)-6 levels were measured by enzyme-linked immunosorbent assay. RESULTS The life span of AKI goats was extended: the lifetime with the BTD treatment compared with sham-BTD. BTD and sham-BTD showed a similar degree of small solute clearance. The expression levels of inflammatory cytokines and plasma IL-6 levels were decreased by the BTD treatment. CONCLUSIONS BTD treatment results in less damage from endotoxin shock and increased life span in AKI goats. These results suggest that BTD may be a useful component of bioartificial kidneys and should be considered in the next generation of renal replacement therapies. |
| Databáze: | OpenAIRE |
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