Comparison of Symbicort® versus Pulmicort® on steroid pharmacodynamic markers in asthma patients
| Autor: | Kazuhiro Ito, Sergei A. Kharitonov, Misako Ito, Peter J. Barnes, Sarah Essilfie-Quaye |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Budesonide Combination therapy Enzyme-Linked Immunosorbent Assay Pharmacology Response Elements Placebo Double-Blind Method Adrenal Cortex Hormones Administration Inhalation medicine Budesonide Formoterol Fumarate Drug Combination Humans Anti-Asthmatic Agents Glucocorticoids Asthma Cross-Over Studies Dose-Response Relationship Drug Inhalation Reverse Transcriptase Polymerase Chain Reaction business.industry Interleukin-8 Sputum Dual Specificity Phosphatase 1 medicine.disease Bronchodilator Agents Drug Combinations Cortosteroid Ethanolamines Pharmacodynamics Sputum macrophage GR-GRE Drug Therapy Combination Female Formoterol medicine.symptom Phramacodynamic marker business Biomarkers medicine.drug |
| Zdroj: | Respiratory Medicine. 105:1784-1789 |
| ISSN: | 0954-6111 0015-9263 |
| DOI: | 10.1016/j.rmed.2011.08.020 |
| Popis: | SummaryBackgroundCombination therapy with inhaled corticosteroids (ICS) and long-acting β2-adrenergic agonists (LABA) is reported to have superior effects on controlling asthma symptoms to ICS alone; however, there is no molecular-based evidence to explain the clinical effects. Here, the effect of the ICS/LABA combination was compared with ICS on glucocorticoid receptor (GR) activation in sputum macrophages.MethodsIn a randomised, double-blind cross-over placebo-controlled 6-visit study, 10 patients with mild asthma were given placebo, formoterol (Oxis® 12 μg), budesonide (Pulmicort® 200 μg :BUD200, or 800 μg :BUD800), or budesonide/formoterol combination (Symbicort®) as a single 100/6 μg (SYM100) or double 200/12 μg (SYM200) dose. Sputum macrophages were separated by plate adhesion from induced sputum. GR binding to the glucocorticoid-response elements on oligonucleotides (GR-GRE binding) was evaluated by ELISA. mRNA expression of MAP-kinase phosphatase (MKP)-1 and IL-8 were measured by quantitative RT-PCR.ResultsGR-GRE binding was significantly increased after treatment with SYM100 (3.5 OD/10 μg protein, median, p |
| Databáze: | OpenAIRE |
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