Elevated expression of urotensin II and its receptor in diabetic cardiomyopathy
| Autor: | Hongyan Dai, Yun Zhang, Xiao-Jing Yu, Zhiming Ge, Meng-Xiong Tang, Xin-Gui Guo, Zhen-Hua Li |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Chronotropic
Male medicine.medical_specialty Heart Diseases Endocrinology Diabetes and Metabolism Urotensins Diabetic angiopathy Diabetes Mellitus Experimental Receptors G-Protein-Coupled chemistry.chemical_compound Endocrinology Internal medicine Diabetic cardiomyopathy Internal Medicine Medicine Animals Rats Wistar Receptor Messenger RNA business.industry Reverse Transcriptase Polymerase Chain Reaction Myocardium Streptozotocin medicine.disease Rats chemistry Gene Expression Regulation cardiovascular system business Urotensin-II Diabetic Angiopathies medicine.drug |
| Zdroj: | Journal of diabetes and its complications. 22(2) |
| ISSN: | 1056-8727 |
| Popis: | Objective Urotensin II (UII) is a somatostatin-like peptide recently identified to have several cardiovascular effects, including potent vasoactive, cardiac inotropic and chronotropic properties. Our aim was to determine the degree of expression of UII and UII receptor (UT) in the myocardium of rats with streptozotocin (STZ)-induced diabetes. Methods Real-time polymerase chain reaction, Western blot, and immunohistochemistry were used to determine the degree of expression and location of UII and UT in the myocardium of STZ-induced diabetic rats. Results UII and UT expression were significantly enhanced in the myocardium of rats with diabetes compared with healthy controls on both messenger RNA and protein levels. Both UII and UT protein expression were mainly concentrated in the cardiomyocytes, endothelial cells, cardiac fibroblasts, and smooth muscle cells of diabetic cardiomyopathy (DCM). Conclusions Our results suggest a possible role for the UII/UT system in the pathophysiology of DCM. |
| Databáze: | OpenAIRE |
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