Characteristic variation of α-fetoprotein DNA nanometer-range irradiated by iodine-125
| Autor: | Liang-jun Xie, Mu-Hua Cheng, Zheng-jie Wu, Jian-fang Li, Yao-xiong Huang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Purine
Cancer Research medicine.disease_cause Spectrum Analysis Raman law.invention Iodine Radioisotopes chemistry.chemical_compound symbols.namesake law Pyridine medicine Humans Radiology Nuclear Medicine and imaging Irradiation Polymerase chain reaction Pharmacology Mutation Radiotherapy General Medicine DNA Genetic Therapy Original Articles Oligonucleotides Antisense Molecular biology Crystallography Oncology chemistry Phosphodiester bond symbols alpha-Fetoproteins Raman spectroscopy DNA Damage |
| Zdroj: | Cancer biotherapyradiopharmaceuticals. 28(3) |
| ISSN: | 1557-8852 |
| Popis: | To obtain the characteristic variation of structure and functional groups of α-fetoprotein (AFP) DNA irradiated by iodine-125((125)I), the AFP antisense oligonucleotide labeled with various radioactivity dose (125)I was mixed with the AFP DNA in a simulated polymerase chain reaction temperature condition. After the mixtures were irradiated by the (125)I from 2 to 72 hours, the mutation of the biogenic conformation and functional groups of the irradiated DNA were investigated using laser Raman spectroscopy. The shifted peak and the decreased intensity of the characteristic Raman spectra were found, which demonstrated that the structure of the phosphodiester linkage was broke, the pyridine and purine bases in DNA emerged and damaged. The model of gene conformation changed from form B to form C spectrum after the nanometer-range irradiation with (125)I from 2 to 24 hours. The damage of local pyridine and purine bases gradually increased along with the accumulation of irradiation, and the bases and ribosome were finally dissociated and stacked. |
| Databáze: | OpenAIRE |
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