miRNA transfection via poly(amidoamine)-based delivery vector prevents hypoxia/reperfusion-induced cardiomyocyte apoptosis
| Autor: | Kishore M. Paknikar, Nida Sayed, Prajakta Tambe, Virendra Gajbhiye, Sachin H Jadhav, Pramod Kumar |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Genetic Vectors
Myocardial Infarction Biomedical Engineering Medicine (miscellaneous) Apoptosis Myocardial Reperfusion Injury Bioengineering 02 engineering and technology Development Transfection 03 medical and health sciences 0302 clinical medicine RNA interference microRNA Polyamines medicine Animals Humans Histidine Myocytes Cardiac General Materials Science Chemistry Gene Transfer Techniques Poly(amidoamine) Hypoxia (medical) 021001 nanoscience & nanotechnology Rats Cell biology MicroRNAs 030220 oncology & carcinogenesis cardiovascular system Nanoparticles medicine.symptom Nanocarriers 0210 nano-technology Cardiomyocyte apoptosis Signal Transduction |
| Zdroj: | Nanomedicine. 15:163-181 |
| ISSN: | 1748-6963 1743-5889 |
| DOI: | 10.2217/nnm-2019-0363 |
| Popis: | Aim: Myocardial infarction is a tissue injury that leads to apoptosis of cardiomyocytes. This can be prevented by using miRNAs, but its delivery to cardiomyocytes is a major hurdle. We aimed to deliver miRNAs using poly(amidoamine)-histidine (PAMAM-His) nanocarriers to prevent apoptosis. Materials & methods: The PAMAM-His nanoparticles were synthesized and assessed for their transfection efficiency of miRNAs to prevent apoptosis in hypoxia/reperfusion-induced H9c2 as well as primary cultured cardiomyocytes. Results & conclusion: miRNAs-nanoparticle complexes exerted a significant antiapoptotic effect on the H9c2 and primary rat ventricular cardiomyocytes. Enhanced expression of antiapoptotic genes and decreased expression of proapoptotic genes were observed. PAMAM-His nanoparticles effectively delivered miRNAs to the cardiomyocytes and prevented the hypoxia/reperfusion-induced apoptosis critical in myocardial infarctions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|