Antimicrobial effect of anacardic acid–loaded zein nanoparticles loaded on Streptococcus mutans biofilms
| Autor: | Ramille Araújo Lima, Ana Larissa Ximenes Batista, Lais Aragão Lima, Francisco Fábio Oliveira de Sousa, Jennifer Thayanne Cavalcante de Araújo, Smyrna Luiza Ximenes de Souza, Juliana Paiva Marques Lima Rolim, Tereza de Jesus Pinheiro Gomes Bandeira |
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| Rok vydání: | 2020 |
| Předmět: |
Zein
Microbial Sensitivity Tests Microbiology Streptococcus mutans 03 medical and health sciences Minimum inhibitory concentration Dry weight Media Technology Food microbiology Anacardium Biotechnology and Industrial Microbiology - Research Paper Food science 030304 developmental biology 0303 health sciences Microbial Viability biology Plant Extracts 030306 microbiology Chemistry Biofilm biology.organism_classification In vitro Anacardic Acids Anti-Bacterial Agents Biofilms Drug delivery Nanoparticles |
| Zdroj: | Braz J Microbiol |
| ISSN: | 1678-4405 1517-8382 |
| DOI: | 10.1007/s42770-020-00320-2 |
| Popis: | Bacterial biofilms play a key role in the pathogenesis of major oral diseases. Nanoparticles open new paths for drug delivery in complex structures such as biofilms. This study evaluated the antimicrobial effect of zein nanoparticles containing anacardic acid (AA) extracted from cashew shells of Anacardium occidentale on in vitro Streptococcus mutans biofilm formation and mature biofilms. The minimum inhibitory concentration (MIC), minimum bacterial concentration (MBC), and antibiofilm assays were performed. Streptococcus mutans UA159 biofilms were formed on saliva-coated hydroxyapatite disk for 5 days. To evaluate the preventive effect on biofilm formation, before contact with the inoculum, the disks were immersed once for 2 min in (1) hydroethanolic solution; (2) blank zein nanoparticles; (3) zein nanoparticles containing AA; and (4) 0.12% chlorhexidine gluconate. To determine the effect against mature biofilms, the disks containing 5-day preformed biofilms were further treated using the same procedure. The bacterial viability and dry weight were determined for both assays and used to compare the groups using ANOVA followed by Tukey’s test (p |
| Databáze: | OpenAIRE |
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